Collectrin, a Collecting Duct-specific Transmembrane Glycoprotein, is a Novel Homolog of ACE2 and is Developmentally Regulated in Embryonic Kidneys

Hong Zhang, Jun Wada, Kazuyuki Hida, Yoshinori Tsuchiyama, Keita Hiragushi, Kenichi Shikata, Haiyan Wang, Sun Lin, Yashpal S. Kanwar, Hirofumi Makino

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Collectrin, a novel homolog of angiotensin-converting enzyme-related carboxypeptidase (ACE2), was identified during polymerase chain reaction-based cDNA subtraction and up-regulated in 5/6 ablated kidneys at hypertrophic phase. Collectrin, with 222 amino acids, has an apparent signal peptide and a transmembrane domain; the sequence is conserved in mouse, rat, and human and shares 81.9% identity. Human collectrin has 47.8% identity with non-catalytic extracellular, transmembrane, and cytosolic domains of ACE2; however, unlike ACE and ACE2, collectrin lacks active dipeptidyl carboxypeptidase catalytic domains. The collectrin mRNA transcripts are expressed exclusively in the kidney. In situ hybridization reveals its mRNA expression in renal collecting ducts, and immunohistochemistry shows that it is localized to the luminal surface and cytoplasm of collecting ducts. Immunoprecipitation studies, using [35S]methionine-labeled renal cortical and inner medullar collecting duct cells, i.e. M-1 and mIMCD-3, indicate that the protein size is ∼32 kDa. During the development of mouse kidney, mRNA signal is detectable at day 13 of gestation, and the protein product is observed in the ureteric bud branches. Its expression is progressively increased during later stages of the gestation extending into the neonatal periods and then is decreased in adult life. Up-regulated expression of collectrin in the hypertrophic kidneys after renal ablation and restricted spatio-temporal expression during development indicates a possible role(s)in the process of progressive renal failure and renal organogenesis.

Original languageEnglish
Pages (from-to)17132-17139
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number20
DOIs
Publication statusPublished - May 18 2001

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Ducts
Glycoproteins
Kidney
Messenger RNA
Polymerase chain reaction
Protein Sorting Signals
Ablation
Methionine
Rats
Proteins
Complementary DNA
Amino Acids
Pregnancy
Organogenesis
Conserved Sequence
Immunoprecipitation
In Situ Hybridization
Renal Insufficiency
Catalytic Domain
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry

Cite this

Collectrin, a Collecting Duct-specific Transmembrane Glycoprotein, is a Novel Homolog of ACE2 and is Developmentally Regulated in Embryonic Kidneys. / Zhang, Hong; Wada, Jun; Hida, Kazuyuki; Tsuchiyama, Yoshinori; Hiragushi, Keita; Shikata, Kenichi; Wang, Haiyan; Lin, Sun; Kanwar, Yashpal S.; Makino, Hirofumi.

In: Journal of Biological Chemistry, Vol. 276, No. 20, 18.05.2001, p. 17132-17139.

Research output: Contribution to journalArticle

Zhang, Hong ; Wada, Jun ; Hida, Kazuyuki ; Tsuchiyama, Yoshinori ; Hiragushi, Keita ; Shikata, Kenichi ; Wang, Haiyan ; Lin, Sun ; Kanwar, Yashpal S. ; Makino, Hirofumi. / Collectrin, a Collecting Duct-specific Transmembrane Glycoprotein, is a Novel Homolog of ACE2 and is Developmentally Regulated in Embryonic Kidneys. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 20. pp. 17132-17139.
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