Collagen XVIII deposition in the basement membrane zone beneath the newly forming epidermis during wound healing in mice

Takahiro Maeba, Tomoko Yonezawa, Mitsuaki Ono, Yasuko Tomono, Ritva Heljasvaara, Taina Pihlajaniemi, Kiichi Inagawa, Toshitaka Oohashi

Research output: Contribution to journalArticle

Abstract

The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of α1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.

Original languageEnglish
Pages (from-to)135-146
Number of pages12
JournalActa medica Okayama
Volume73
Issue number2
Publication statusPublished - Jan 1 2019

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Collagen Type XVIII
Basement Membrane
Epidermis
Wound Healing
Re-Epithelialization
Wounds and Injuries
Protein Isoforms
Tissue regeneration
Skin
Regeneration
Collagen
Immunohistochemistry
Maintenance

Keywords

  • Basement membrane
  • Collagen XVIII
  • Re-epithelialization
  • Skin
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Collagen XVIII deposition in the basement membrane zone beneath the newly forming epidermis during wound healing in mice. / Maeba, Takahiro; Yonezawa, Tomoko; Ono, Mitsuaki; Tomono, Yasuko; Heljasvaara, Ritva; Pihlajaniemi, Taina; Inagawa, Kiichi; Oohashi, Toshitaka.

In: Acta medica Okayama, Vol. 73, No. 2, 01.01.2019, p. 135-146.

Research output: Contribution to journalArticle

Maeba, Takahiro ; Yonezawa, Tomoko ; Ono, Mitsuaki ; Tomono, Yasuko ; Heljasvaara, Ritva ; Pihlajaniemi, Taina ; Inagawa, Kiichi ; Oohashi, Toshitaka. / Collagen XVIII deposition in the basement membrane zone beneath the newly forming epidermis during wound healing in mice. In: Acta medica Okayama. 2019 ; Vol. 73, No. 2. pp. 135-146.
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