Abstract
The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of α1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.
| Original language | English |
|---|---|
| Pages (from-to) | 135-146 |
| Number of pages | 12 |
| Journal | Acta medica Okayama |
| Volume | 73 |
| Issue number | 2 |
| Publication status | Published - Jan 1 2019 |
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Keywords
- Basement membrane
- Collagen XVIII
- Re-epithelialization
- Skin
- Wound healing
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Collagen XVIII deposition in the basement membrane zone beneath the newly forming epidermis during wound healing in mice. / Maeba, Takahiro; Yonezawa, Tomoko; Ono, Mitsuaki; Tomono, Yasuko; Heljasvaara, Ritva; Pihlajaniemi, Taina; Inagawa, Kiichi; Oohashi, Toshitaka.
In: Acta medica Okayama, Vol. 73, No. 2, 01.01.2019, p. 135-146.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Collagen XVIII deposition in the basement membrane zone beneath the newly forming epidermis during wound healing in mice
AU - Maeba, Takahiro
AU - Yonezawa, Tomoko
AU - Ono, Mitsuaki
AU - Tomono, Yasuko
AU - Heljasvaara, Ritva
AU - Pihlajaniemi, Taina
AU - Inagawa, Kiichi
AU - Oohashi, Toshitaka
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of α1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.
AB - The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of α1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.
KW - Basement membrane
KW - Collagen XVIII
KW - Re-epithelialization
KW - Skin
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85064631716&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064631716&partnerID=8YFLogxK
M3 - Article
C2 - 31015748
AN - SCOPUS:85064631716
VL - 73
SP - 135
EP - 146
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 2
ER -