Collagen XVIII, a basement membrane heparan sulfate proteoglycan, interacts with L-selectin and monocyte chemoattractant protein-1

Hiroto Kawashima, Norifumi Watanabe, Mayumi Hirose, Xin Sun, Kazuyuki Atarashi, Tetsuya Kimura, Kenichi Shikata, Mitsuhiro Matsuda, Daisuke Ogawa, Ritva Heljasvaara, Marko Rehn, Taina Pihlajaniemi, Masayuki Miyasaka

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Leukocyte infiltration during inflammation is mediated by the sequential actions of adhesion molecules and chemokines. By using a rat ureteral obstruction model, we showed previously that L-selectin plays an important role in leukocyte infiltration into the kidney. Here we report the purification, identification, and characterization of an L-selectin-binding heparan sulfate proteoglycan (HSPG) expressed in the rat kidney. Partial amino acid sequencing and Western blotting analyses showed that the L-selectin-binding HSPG is collagen XVIII, a basement membrane HSPG. The binding of L-selectin to isolated collagen XVIII was specifically inhibited by an anti-L-selectin monoclonal antibody, EDTA, treatment of the collagen XVIII with heparitinase or heparin but not by chemically desulfated heparin. A cell binding assay showed that the L-selectin-collagen XVIII interaction mediates cell adhesion. Interestingly, collagen XVIII also interacted with a chemokine, monocyte chemoattractant protein-1, and presented it to a monocytic cell line, THP-1, which enhanced the α4β1 integrin-mediated binding of the THP-1 cells to vascular cell adhesion molecule-1. Thus, collagen XVIII may provide a link between selectin-mediated cell adhesion and chemokine-induced cellular activation and accelerate the progression of leukocyte infiltration in renal inflammation.

Original languageEnglish
Pages (from-to)13069-13076
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number15
DOIs
Publication statusPublished - Apr 11 2003

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Collagen Type XVIII
L-Selectin
Heparan Sulfate Proteoglycans
Chemokine CCL2
Basement Membrane
Chemokines
Infiltration
Leukocytes
Cell adhesion
heparitinsulfate lyase
Kidney
Cell Adhesion
Heparin
Rats
Inflammation
Selectins
Ureteral Obstruction
Vascular Cell Adhesion Molecule-1
Protein Sequence Analysis
Integrins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Collagen XVIII, a basement membrane heparan sulfate proteoglycan, interacts with L-selectin and monocyte chemoattractant protein-1. / Kawashima, Hiroto; Watanabe, Norifumi; Hirose, Mayumi; Sun, Xin; Atarashi, Kazuyuki; Kimura, Tetsuya; Shikata, Kenichi; Matsuda, Mitsuhiro; Ogawa, Daisuke; Heljasvaara, Ritva; Rehn, Marko; Pihlajaniemi, Taina; Miyasaka, Masayuki.

In: Journal of Biological Chemistry, Vol. 278, No. 15, 11.04.2003, p. 13069-13076.

Research output: Contribution to journalArticle

Kawashima, H, Watanabe, N, Hirose, M, Sun, X, Atarashi, K, Kimura, T, Shikata, K, Matsuda, M, Ogawa, D, Heljasvaara, R, Rehn, M, Pihlajaniemi, T & Miyasaka, M 2003, 'Collagen XVIII, a basement membrane heparan sulfate proteoglycan, interacts with L-selectin and monocyte chemoattractant protein-1', Journal of Biological Chemistry, vol. 278, no. 15, pp. 13069-13076. https://doi.org/10.1074/jbc.M212244200
Kawashima, Hiroto ; Watanabe, Norifumi ; Hirose, Mayumi ; Sun, Xin ; Atarashi, Kazuyuki ; Kimura, Tetsuya ; Shikata, Kenichi ; Matsuda, Mitsuhiro ; Ogawa, Daisuke ; Heljasvaara, Ritva ; Rehn, Marko ; Pihlajaniemi, Taina ; Miyasaka, Masayuki. / Collagen XVIII, a basement membrane heparan sulfate proteoglycan, interacts with L-selectin and monocyte chemoattractant protein-1. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 15. pp. 13069-13076.
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AU - Sun, Xin

AU - Atarashi, Kazuyuki

AU - Kimura, Tetsuya

AU - Shikata, Kenichi

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AU - Ogawa, Daisuke

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AU - Pihlajaniemi, Taina

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