Collagen-like antimicrobial peptides

Ryo Masuda, Masakazu Kudo, Yui Dazai, Takehiko Mima, Takaki Koide

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Combinatorial library composed of rigid rod-like peptides with a triple-helical scaffold was constructed. The component peptides were designed to have various combinations of basic and neutral (or hydrophobic) amino acid residues based on collagen-like (Gly-Pro-Yaa)-repeating sequences, inspired from the basic and amphiphilic nature of naturally occurring antimicrobial peptides. Screening of the peptide pools resulted in identification of antimicrobial peptides. A structure-activity relationship study revealed that the position of Arg-cluster at N-terminus and cystine knots at C-terminus in the triple helix significantly contributed to the antimicrobial activity. The most potent peptide RO-A showed activity against Gram-negative Escherichia coli and Gram-positive Bacillus subtilis. In addition, Escherichia coli exposed to RO-A resulted in abnormal elongation of the cells. RO-A was also shown to have remarkable stability in human serum and low cytotoxicity to mammalian cells.

Original languageEnglish
Pages (from-to)453-459
Number of pages7
JournalBiopolymers
DOIs
Publication statusPublished - Nov 4 2016

Fingerprint

Collagen
Peptides
Escherichia coli
glycylproline
Cystines
Cystine
Bacilli
Structure-Activity Relationship
Scaffolds (biology)
Cytotoxicity
Bacillus subtilis
Scaffolds
Amino acids
Elongation
Screening
Cells
Amino Acids
Serum

Keywords

  • antimicrobial peptide
  • collagen
  • combinatorial library
  • structure-activity relationship
  • triple helix

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biomaterials
  • Organic Chemistry

Cite this

Masuda, R., Kudo, M., Dazai, Y., Mima, T., & Koide, T. (2016). Collagen-like antimicrobial peptides. Biopolymers, 453-459. https://doi.org/10.1002/bip.22791

Collagen-like antimicrobial peptides. / Masuda, Ryo; Kudo, Masakazu; Dazai, Yui; Mima, Takehiko; Koide, Takaki.

In: Biopolymers, 04.11.2016, p. 453-459.

Research output: Contribution to journalArticle

Masuda, R, Kudo, M, Dazai, Y, Mima, T & Koide, T 2016, 'Collagen-like antimicrobial peptides', Biopolymers, pp. 453-459. https://doi.org/10.1002/bip.22791
Masuda R, Kudo M, Dazai Y, Mima T, Koide T. Collagen-like antimicrobial peptides. Biopolymers. 2016 Nov 4;453-459. https://doi.org/10.1002/bip.22791
Masuda, Ryo ; Kudo, Masakazu ; Dazai, Yui ; Mima, Takehiko ; Koide, Takaki. / Collagen-like antimicrobial peptides. In: Biopolymers. 2016 ; pp. 453-459.
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