Collagen-binding domain of a Clostridium histolyticum collagenase exhibits a broad substrate spectrum both in vitro and in vivo

T. Toyoshima, Osamu Matsushita, J. Minami, N. Nishi, A. Okabe, T. Itano

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The substrate spectrum of the tandem collagen-binding domain (CBD) of Clostridium histolyticumclass I collagenase (ColG) was examined both in vitro and in vivo. CBD bound to insoluble type I, II, III and IV collagens in vitro, and to skin, aorta, tendon, kidney, trachea and corneal tissues containing various types of collagen fibrils or sheets. CBD bound to all kinds of collagen fibrils regardless of their diameters and also bound to sheet-forming collagen in the glomerular basal lamina or Descemet's membrane of the cornea. This wide substrate spectrum expands possible applications of the drug delivery system we proposed previously (PNAS 95:7018-7023, 1998). Therapeutic agents fused with CBD will bind not only to subcutaneous tissues, but also to other tissues containing non-type I collagen.

Original languageEnglish
Pages (from-to)281-290
Number of pages10
JournalConnective Tissue Research
Volume42
Issue number4
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Microbial Collagenase
Collagen
Substrates
Tissue
Descemet Membrane
Collagen Type IV
Collagen Type II
Clostridium
In Vitro Techniques
Subcutaneous Tissue
Collagenases
Drug Delivery Systems
Collagen Type I
Trachea
Tendons
Basement Membrane
Cornea
Aorta
Skin
Kidney

Keywords

  • Clostridium histolyticum
  • Collagen-binding domain
  • Collagenase
  • Immunoelectron microscopy
  • Substrate spectrum

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Nephrology
  • Cell Biology
  • Orthopedics and Sports Medicine

Cite this

Collagen-binding domain of a Clostridium histolyticum collagenase exhibits a broad substrate spectrum both in vitro and in vivo. / Toyoshima, T.; Matsushita, Osamu; Minami, J.; Nishi, N.; Okabe, A.; Itano, T.

In: Connective Tissue Research, Vol. 42, No. 4, 2001, p. 281-290.

Research output: Contribution to journalArticle

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