TY - JOUR
T1 - Collagen adhesion gene is associated with bloodstream infections caused by methicillin-resistant Staphylococcus aureus
AU - Iwata, Yasunori
AU - Satou, Kenji
AU - Furuichi, Kengo
AU - Yoneda, Ikuko
AU - Matsumura, Takuhiro
AU - Yutani, Masahiro
AU - Fujinaga, Yukako
AU - Hase, Atsushi
AU - Morita, Hidetoshi
AU - Ohta, Toshiko
AU - Senda, Yasuko
AU - Sakai-Takemori, Yukiko
AU - Wada, Taizo
AU - Fujita, Shinichi
AU - Miyake, Taito
AU - Yasuda, Haruka
AU - Sakai, Norihiko
AU - Kitajima, Shinji
AU - Toyama, Tadashi
AU - Shinozaki, Yasuyuki
AU - Sagara, Akihiro
AU - Miyagawa, Taro
AU - Hara, Akinori
AU - Shimizu, Miho
AU - Kamikawa, Yasutaka
AU - Ikeo, Kazuho
AU - Shichino, Shigeyuki
AU - Ueha, Satoshi
AU - Nakajima, Takuya
AU - Matsushima, Kouji
AU - Kaneko, Shuichi
AU - Wada, Takashi
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research on Innovative Areas program (Inflammation Cellular Sociology, 17H06394, YI, NS, KF and TW) and JSPS KAKENHI (18K08426, YI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research on Innovative Areas program (Inflammation Cellular Sociology, 17H06394 , YI, NS, KF and TW) and JSPS KAKENHI ( 18K08426 , YI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2019 The Author(s)
PY - 2020/2
Y1 - 2020/2
N2 - Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) causes hospital- and community-acquired infections. It is not clear whether genetic characteristics of the bacteria contribute to disease pathogenesis in MRSA infection. We hypothesized that whole genome analysis of MRSA strains could reveal the key gene loci and/or the gene mutations that affect clinical manifestations of MRSA infection. Methods: Whole genome sequences (WGS) of MRSA of 154 strains were analyzed with respect to clinical manifestations and data. Further, we evaluated the association between clinical manifestations in MRSA infection and genomic information. Results: WGS revealed gene mutations that correlated with clinical manifestations of MRSA infection. Moreover, 12 mutations were selected as important mutations by Random Forest analysis. Cluster analysis revealed strains associated with a high frequency of bloodstream infection (BSI). Twenty seven out of 34 strains in this cluster caused BSI. These strains were all positive for collagen adhesion gene (cna) and have mutations in the locus, those were selected by Random Forest analysis. Univariate and multivariate analysis revealed that these gene mutations were the predictor for the incidence of BSI. Interestingly, mutant CNA protein showed lower attachment ability to collagen, suggesting that the mutant protein might contribute to the dissemination of bacteria. Conclusions: These findings suggest that the bacterial genotype affects the clinical characteristics of MRSA infection.
AB - Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) causes hospital- and community-acquired infections. It is not clear whether genetic characteristics of the bacteria contribute to disease pathogenesis in MRSA infection. We hypothesized that whole genome analysis of MRSA strains could reveal the key gene loci and/or the gene mutations that affect clinical manifestations of MRSA infection. Methods: Whole genome sequences (WGS) of MRSA of 154 strains were analyzed with respect to clinical manifestations and data. Further, we evaluated the association between clinical manifestations in MRSA infection and genomic information. Results: WGS revealed gene mutations that correlated with clinical manifestations of MRSA infection. Moreover, 12 mutations were selected as important mutations by Random Forest analysis. Cluster analysis revealed strains associated with a high frequency of bloodstream infection (BSI). Twenty seven out of 34 strains in this cluster caused BSI. These strains were all positive for collagen adhesion gene (cna) and have mutations in the locus, those were selected by Random Forest analysis. Univariate and multivariate analysis revealed that these gene mutations were the predictor for the incidence of BSI. Interestingly, mutant CNA protein showed lower attachment ability to collagen, suggesting that the mutant protein might contribute to the dissemination of bacteria. Conclusions: These findings suggest that the bacterial genotype affects the clinical characteristics of MRSA infection.
KW - Bloodstream infection
KW - Cna
KW - MRSA
KW - Whole genome sequencing
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U2 - 10.1016/j.ijid.2019.11.003
DO - 10.1016/j.ijid.2019.11.003
M3 - Article
C2 - 31740408
AN - SCOPUS:85077546539
SN - 1201-9712
VL - 91
SP - 22
EP - 31
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -
