Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence

Juan M. Arjona-López, Paul Telengech, Nobuhiro Suzuki, Carlos J. López-Herrera

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Many Mediteranean isolates of Rosellinia necatrix, causing root rot in avocado, have previously been screened for viruses. Among them is isolate Rn459 that has been shown to be infected by at least three viruses such as Rosellinia necatrix partitivirus 10 (RnPV10), Rosellinia necatrix hypovirus 2 (RnHV2), Rosellinia necatrix fusagravirus 1 (RnFGV1), and Rosellinia necatrix virga-like virus (RnVLV). Here, we attempted to eliminate the viruses by hyphal tip cultures to examine their effect on colony growth in vitro and virulence on avocado plants. The obtained fungal strain termed, Rn459_PV10F/VLVF, which was confirmed to be cured of RnPV10 and RnVLV, but still retaining RnHV2, manifested a phenotype different from the original Rn459. Colony growth comparison showed that Rn459_PV10F/VLVF grew faster than the original Rn459 isolate and the virulence on avocado plants of this Rn459_PV10F/VLVF strain was higher than the original Rn459 strain. These combined results suggest that RnPV10 and RnVLV, alone or together, contribute to confer hypovirulence on the R. necatrix isolates.

    Original languageEnglish
    Pages (from-to)111-119
    Number of pages9
    JournalEuropean Journal of Plant Pathology
    Volume158
    Issue number1
    DOIs
    Publication statusPublished - Sep 1 2020

    Keywords

    • Curing
    • Hypovirulence
    • Mycovirus
    • Partitivirus
    • Persea americana;virocontrol

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Plant Science
    • Horticulture

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