Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres

Kiyotaka Nagaki, Fukashi Shibata, Go Suzuki, Asaka Kanatani, Souichi Ozaki, Akiko Hironaka, Kazunari Kashihara, Minoru Murata

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although a centromeric DNA fragment of tobacco (Nicotiana tabacum), Nt2-7, has been reported, the overall structure of the centromeres remains unknown. To characterize the centromeric DNA sequences, we conducted a chromatin immunoprecipitation assay using anti-NtCENH3 antibody and chromatins isolated from two ancestral diploid species (Nicotiana sylvestris and Nicotiana tomentosiformis) of N. tabacum and isolated a 178-pb fragment, Nto1 from N. tomentosiformis, as a novel centromeric DNA. Fluorescence in situ hybridization (FISH) showed that Nto1 localizes on 24 out of 48 chromosomes in some cells of a BY-2 cell line. To identify the origins of the Nt2-7 and Nto1, a tobacco bacterial artificial chromosome (BAC) library was constructed from N. tabacum, and then screened by polymerase chain reaction (PCR) with primer sets designed from the Nt2-7 and Not1 DNA sequences. Twelve BAC clones were found to localize on the centromeric regions by FISH. We selected three BAC clones for sequencing and identified two centromeric retrotransposons, NtCR and NtoCR, the DNA sequences of which are similar to that of Nt2-7 and Nto1, respectively. Quantitative PCR analysis using coprecipitated DNA with anti-NtCENH3 clearly showed coexistence of NtCENH3 with both retrotransposons. These results indicate the possibility that these two retrotransposons act as centromeric DNA sequences in tobacco. NtoCR was found to be specific to N. tomentosiformis and T genome of N. tabacum, and a NtCR-like centromeric retrotransposon (TGRIV) exists in tomato. This specificity suggests that the times of amplification of these centromeric retrotransposons were different.

Original languageEnglish
Pages (from-to)591-605
Number of pages15
JournalChromosome Research
Volume19
Issue number5
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Retroelements
Centromere
Tobacco
Bacterial Artificial Chromosomes
Fluorescence In Situ Hybridization
DNA
Clone Cells
Polymerase Chain Reaction
Chromatin Immunoprecipitation
Lycopersicon esculentum
Diploidy
Chromatin
Anti-Idiotypic Antibodies
Chromosomes
Genome
Cell Line

Keywords

  • BAC
  • centromere
  • chromatin immunoprecipitation
  • Nicotiana
  • retrotransposon
  • tobacco

ASJC Scopus subject areas

  • Genetics

Cite this

Nagaki, K., Shibata, F., Suzuki, G., Kanatani, A., Ozaki, S., Hironaka, A., ... Murata, M. (2011). Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres. Chromosome Research, 19(5), 591-605. https://doi.org/10.1007/s10577-011-9219-2

Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres. / Nagaki, Kiyotaka; Shibata, Fukashi; Suzuki, Go; Kanatani, Asaka; Ozaki, Souichi; Hironaka, Akiko; Kashihara, Kazunari; Murata, Minoru.

In: Chromosome Research, Vol. 19, No. 5, 07.2011, p. 591-605.

Research output: Contribution to journalArticle

Nagaki, K, Shibata, F, Suzuki, G, Kanatani, A, Ozaki, S, Hironaka, A, Kashihara, K & Murata, M 2011, 'Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres', Chromosome Research, vol. 19, no. 5, pp. 591-605. https://doi.org/10.1007/s10577-011-9219-2
Nagaki, Kiyotaka ; Shibata, Fukashi ; Suzuki, Go ; Kanatani, Asaka ; Ozaki, Souichi ; Hironaka, Akiko ; Kashihara, Kazunari ; Murata, Minoru. / Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres. In: Chromosome Research. 2011 ; Vol. 19, No. 5. pp. 591-605.
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