Cochlear expression of a dominant-negative GJB2R75W construct delivered through the round window membrane in mice

Yukihide Maeda, Kunihiro Fukushima, Akihiro Kawasaki, Kazunori Nishizaki, Richard J.H. Smith

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Development of a gene-delivery method to the inner ear is an essential step for studies of hearing function and gene therapy. Application of liposomes or adenoviral vectors onto the intact round window membrane (RWM) offers the possibility of atraumatic exogenous gene transfer. GJB2 encodes the gap junction protein Connexin26, which plays a crucial role in potassium recycling in the inner ear. The R75W allele of GJB is a well-characterized mutation that causes deafness at the DFNA3 through a dominant-negative mechanism of action. In this study, a plasmid vector, pGJB2R75W-eGFP, was lipocomplexed with N-[1-(2,3-Dioleoloxy)propyl]N,N,N-trimethylammonium methylsulfate: cholesterol and applied onto mouse RWM. At 3 days (3 d) post-treatment, immunohistochemistry demonstrated GJB2R75W-eGFP transgene expression in the cochlea in: inner and outer pillar cells, outer hair cells, Claudius cells and, in the spiral limbus and ligament. Significant hearing loss was detected by auditory brainstem response testing after 1, 2 and 3 d post-treatment; hearing levels returned to control levels at 5 d post-treatment. These data confirm that GJB2R75W induces functional impairment in the mature cochlea through a dominant negative effect, and importantly, that RWM application of exogenous genes is a feasible method to test their impact on hearing.

Original languageEnglish
Pages (from-to)250-254
Number of pages5
JournalNeuroscience Research
Volume58
Issue number3
DOIs
Publication statusPublished - Jul 2007

Keywords

  • Auditory brainstem response
  • DOTAP
  • Dominant negative effect
  • GJB2
  • Immunohistochemistry
  • Round window membrane

ASJC Scopus subject areas

  • Neuroscience(all)

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