Clues to diagnosis for unusual mucosal pemphigus demonstrating undetectable anti-desmoglein 3 serum antibodies by routine tests

Koji Kamiya, Yumi Aoyama, Mari Yamaguchi, Aya Ukida, Kazuko Mizuno-Ikeda, Kazuyasu Fujii, Toshihisa Hamada, Yoshiki Tokura, Keiji Iwatsuki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pemphigus is an autoimmune blistering disease caused by immunoglobulin (Ig)G autoantibodies against desmogleins (Dsg). In mucosal-dominant pemphigus vulgaris (PV), anti-Dsg3 antibodies play a critical role in acantholysis. We followed two mucosal-dominant PV cases who suffered from refractory oral mucosal erosions. In these cases, anti-Dsg3 serum antibodies were not detected by indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA). However, direct immunofluorescence showed the intercellular IgG deposition in the epidermis and histopathological findings revealed suprabasal acantholysis. In order to analyze the pathomechanisms in these cases, we first examined the Dsg3 expression patterns in lesional sites and compared them with those of typical mucosal-dominant PV cases. In typical PV cases, the alteration of Dsg3 distribution was observed in lesional sites by immunostaining. The aggregation of Dsg3, which is the characteristic change in PV mucosal lesions, was observed as the initial change prior to acantholysis. In our cases, a clustering of Dsg3 was observed at mucosal lesions, and the expression levels of Dsg3 in acantholytic lesions were decreased, as observed in typical mucosal-dominant PV cases. Although anti-Dsg3 serum antibodies could not be detected by routine tests, anti-Dsg3 serum antibodies were detected by Dsg3 ELISA using 10-times more concentrated sera (highly sensitive ELISA). Moreover, purified and concentrated PV IgG showed high pathogenicity when examined by dissociation assay. In conclusion, the detection of morphological changes in Dsg3 distribution and highly sensitive ELISA method could be useful for the early diagnosis of PV recurrence.

Original languageEnglish
Pages (from-to)572-579
Number of pages8
JournalJournal of Dermatology
Volume42
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Desmoglein 3
Pemphigus
Antibodies
Serum
Acantholysis
Enzyme-Linked Immunosorbent Assay
Immunoglobulin G
Desmogleins
Direct Fluorescent Antibody Technique
Indirect Fluorescent Antibody Technique
Epidermis
Autoantibodies
Autoimmune Diseases
Cluster Analysis
Virulence
Early Diagnosis
Anti-Idiotypic Antibodies

Keywords

  • anti-desmoglein 3 antibody
  • desmoglein 3 distribution
  • highly sensitive enzyme-linked immunoassay
  • immunostaining
  • pemphigus vulgaris

ASJC Scopus subject areas

  • Dermatology

Cite this

Clues to diagnosis for unusual mucosal pemphigus demonstrating undetectable anti-desmoglein 3 serum antibodies by routine tests. / Kamiya, Koji; Aoyama, Yumi; Yamaguchi, Mari; Ukida, Aya; Mizuno-Ikeda, Kazuko; Fujii, Kazuyasu; Hamada, Toshihisa; Tokura, Yoshiki; Iwatsuki, Keiji.

In: Journal of Dermatology, Vol. 42, No. 6, 01.06.2015, p. 572-579.

Research output: Contribution to journalArticle

Kamiya, K, Aoyama, Y, Yamaguchi, M, Ukida, A, Mizuno-Ikeda, K, Fujii, K, Hamada, T, Tokura, Y & Iwatsuki, K 2015, 'Clues to diagnosis for unusual mucosal pemphigus demonstrating undetectable anti-desmoglein 3 serum antibodies by routine tests', Journal of Dermatology, vol. 42, no. 6, pp. 572-579. https://doi.org/10.1111/1346-8138.12872
Kamiya, Koji ; Aoyama, Yumi ; Yamaguchi, Mari ; Ukida, Aya ; Mizuno-Ikeda, Kazuko ; Fujii, Kazuyasu ; Hamada, Toshihisa ; Tokura, Yoshiki ; Iwatsuki, Keiji. / Clues to diagnosis for unusual mucosal pemphigus demonstrating undetectable anti-desmoglein 3 serum antibodies by routine tests. In: Journal of Dermatology. 2015 ; Vol. 42, No. 6. pp. 572-579.
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