Clostridium botulinum type E toxins bind to Caco-2 cells by a different mechanism from that of type a toxins

Kai Zhang, Yumiko Yamamoto, Tomonori Suzuki, Kenji Yokota, Shaobo Ma, Ni Nengah Dwi Fatmawati, Keiji Oguma

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (He). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or He. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E He significantly inhibited the 7S toxin binding, indicating that He might be a main binding domain of the type E toxin.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalActa Medica Okayama
Volume66
Issue number3
Publication statusPublished - 2012

Fingerprint

Clostridium botulinum type E
Clostridium
Caco-2 Cells
Botulinum Toxins
Hemagglutinins
Clostridium botulinum
Neurotoxins
Cultured Cells
Peptide Hydrolases
Immunoglobulin G
Western Blotting
Light
Dimers
Cells
botulinum toxin type E

Keywords

  • Binding
  • Caco-2
  • Chslridum bolulinum
  • HC
  • Neurotoxins

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Clostridium botulinum type E toxins bind to Caco-2 cells by a different mechanism from that of type a toxins. / Zhang, Kai; Yamamoto, Yumiko; Suzuki, Tomonori; Yokota, Kenji; Ma, Shaobo; Fatmawati, Ni Nengah Dwi; Oguma, Keiji.

In: Acta Medica Okayama, Vol. 66, No. 3, 2012, p. 253-261.

Research output: Contribution to journalArticle

Zhang, Kai ; Yamamoto, Yumiko ; Suzuki, Tomonori ; Yokota, Kenji ; Ma, Shaobo ; Fatmawati, Ni Nengah Dwi ; Oguma, Keiji. / Clostridium botulinum type E toxins bind to Caco-2 cells by a different mechanism from that of type a toxins. In: Acta Medica Okayama. 2012 ; Vol. 66, No. 3. pp. 253-261.
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