Cloning, sequencing and expression of the gene for α antigen from mycobacterium intracellulare and use of PCR for the rapid identification of mycobacterium intracellulare

Hideki Kitaura; Naoya Ohara; Takemitsu Matsuo; Hiromichi Tasaka; Kazuhide Kobayashi; Takeshi Yamada

doi:10.1006/bbrc.1993.2417

Cloning, sequencing and expression of the gene for α antigen from mycobacterium intracellulare and use of PCR for the rapid identification of mycobacterium intracellulare

Hideki Kitaura, Naoya Ohara, Takemitsu Matsuo, Hiromichi Tasaka, Kazuhide Kobayashi, Takeshi Yamada

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.

Original language	English
Pages (from-to)	1466-1473
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	196
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.2417
Publication status	Published - Nov 15 1993
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Cloning, sequencing and expression of the gene for α antigen from mycobacterium intracellulare and use of PCR for the rapid identification of mycobacterium intracellulare. / Kitaura, Hideki; Ohara, Naoya; Matsuo, Takemitsu; Tasaka, Hiromichi; Kobayashi, Kazuhide; Yamada, Takeshi.

In: Biochemical and Biophysical Research Communications, Vol. 196, No. 3, 15.11.1993, p. 1466-1473.

Research output: Contribution to journal › Article › peer-review

Kitaura, H, Ohara, N, Matsuo, T, Tasaka, H, Kobayashi, K & Yamada, T 1993, 'Cloning, sequencing and expression of the gene for α antigen from mycobacterium intracellulare and use of PCR for the rapid identification of mycobacterium intracellulare', Biochemical and Biophysical Research Communications, vol. 196, no. 3, pp. 1466-1473. https://doi.org/10.1006/bbrc.1993.2417

Kitaura, Hideki ; Ohara, Naoya ; Matsuo, Takemitsu ; Tasaka, Hiromichi ; Kobayashi, Kazuhide ; Yamada, Takeshi. / Cloning, sequencing and expression of the gene for α antigen from mycobacterium intracellulare and use of PCR for the rapid identification of mycobacterium intracellulare. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 196, No. 3. pp. 1466-1473.

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