TY - JOUR
T1 - Cloning, sequencing and expression of the gene for α antigen from mycobacterium intracellulare and use of PCR for the rapid identification of mycobacterium intracellulare
AU - Kitaura, Hideki
AU - Ohara, Naoya
AU - Matsuo, Takemitsu
AU - Tasaka, Hiromichi
AU - Kobayashi, Kazuhide
AU - Yamada, Takeshi
PY - 1993/11/15
Y1 - 1993/11/15
N2 - The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.
AB - The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.
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U2 - 10.1006/bbrc.1993.2417
DO - 10.1006/bbrc.1993.2417
M3 - Article
C2 - 8250904
AN - SCOPUS:0027368779
VL - 196
SP - 1466
EP - 1473
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -