Cloning, Sequencing and Expression of the Gene for α Antigen from Mycobacterium intracellulare and Use of PCR for the Rapid Identification of Mycobacterium intracellulare

H. Kitaura, Naoya Oohara, T. Matsuo, H. Tasaka, K. Kobayashi, T. Yamada

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.

Original languageEnglish
Pages (from-to)1466-1473
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number3
DOIs
Publication statusPublished - Nov 15 1993
Externally publishedYes

Fingerprint

Mycobacterium avium Complex
Cloning
Polymerase chain reaction
Organism Cloning
Genes
Gene Expression
Antigens
Amino Acids
Polymerase Chain Reaction
Escherichia coli
Molecular mass
Protein Sorting Signals
Nucleotides
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Cloning, Sequencing and Expression of the Gene for α Antigen from Mycobacterium intracellulare and Use of PCR for the Rapid Identification of Mycobacterium intracellulare. / Kitaura, H.; Oohara, Naoya; Matsuo, T.; Tasaka, H.; Kobayashi, K.; Yamada, T.

In: Biochemical and Biophysical Research Communications, Vol. 196, No. 3, 15.11.1993, p. 1466-1473.

Research output: Contribution to journalArticle

@article{c9372cc811ad4c37b4ce596f8231040d,
title = "Cloning, Sequencing and Expression of the Gene for α Antigen from Mycobacterium intracellulare and Use of PCR for the Rapid Identification of Mycobacterium intracellulare",
abstract = "The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.",
author = "H. Kitaura and Naoya Oohara and T. Matsuo and H. Tasaka and K. Kobayashi and T. Yamada",
year = "1993",
month = "11",
day = "15",
doi = "10.1006/bbrc.1993.2417",
language = "English",
volume = "196",
pages = "1466--1473",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Cloning, Sequencing and Expression of the Gene for α Antigen from Mycobacterium intracellulare and Use of PCR for the Rapid Identification of Mycobacterium intracellulare

AU - Kitaura, H.

AU - Oohara, Naoya

AU - Matsuo, T.

AU - Tasaka, H.

AU - Kobayashi, K.

AU - Yamada, T.

PY - 1993/11/15

Y1 - 1993/11/15

N2 - The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.

AB - The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=0027368779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027368779&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1993.2417

DO - 10.1006/bbrc.1993.2417

M3 - Article

VL - 196

SP - 1466

EP - 1473

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -