Cloning, Sequencing and Expression of the Gene for α Antigen from Mycobacterium intracellulare and Use of PCR for the Rapid Identification of Mycobacterium intracellulare

H. Kitaura, Naoya Oohara, T. Matsuo, H. Tasaka, K. Kobayashi, T. Yamada

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The complete nucleotide sequence of α antigen secreted from Mycobacteriun intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction(PCR) was demonstrated.

Original languageEnglish
Pages (from-to)1466-1473
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number3
DOIs
Publication statusPublished - Nov 15 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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