Abstract
The gene for the extracellular α antigen of Mycobacterium scrofulaceum (S-α) was cloned by using the α antigen gene fragments of Mycobacterium bovis BCG as probes. The complete nucleotide sequence was determined. The gene was expressed in Escherichia coil. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein. The deduced amino acid sequences were highly homologous to the a: antigen of the species of other mycobacteria. Interestingly, the a antigens of MAIS complex (Mycobacterium avium-Mycobacterium intracellulare-M.scrofulaceum) were closely homologous even at the C-terminal regions which were variable among those of M. bovis BCG, Mycobacterium leprae and Mycobacterium kansasii.
| Original language | English |
|---|---|
| Pages (from-to) | 165-170 |
| Number of pages | 6 |
| Journal | Scandinavian Journal of Immunology |
| Volume | 40 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Cloning, sequencing and expression in Escherichia coli of the gene for α antigen from Mycobacterium scrofulaceum. / Takano, M.; Oohara, Naoya; Mizuno, A.; Yamada, T.
In: Scandinavian Journal of Immunology, Vol. 40, No. 2, 1994, p. 165-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning, sequencing and expression in Escherichia coli of the gene for α antigen from Mycobacterium scrofulaceum
AU - Takano, M.
AU - Oohara, Naoya
AU - Mizuno, A.
AU - Yamada, T.
PY - 1994
Y1 - 1994
N2 - The gene for the extracellular α antigen of Mycobacterium scrofulaceum (S-α) was cloned by using the α antigen gene fragments of Mycobacterium bovis BCG as probes. The complete nucleotide sequence was determined. The gene was expressed in Escherichia coil. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein. The deduced amino acid sequences were highly homologous to the a: antigen of the species of other mycobacteria. Interestingly, the a antigens of MAIS complex (Mycobacterium avium-Mycobacterium intracellulare-M.scrofulaceum) were closely homologous even at the C-terminal regions which were variable among those of M. bovis BCG, Mycobacterium leprae and Mycobacterium kansasii.
AB - The gene for the extracellular α antigen of Mycobacterium scrofulaceum (S-α) was cloned by using the α antigen gene fragments of Mycobacterium bovis BCG as probes. The complete nucleotide sequence was determined. The gene was expressed in Escherichia coil. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein. The deduced amino acid sequences were highly homologous to the a: antigen of the species of other mycobacteria. Interestingly, the a antigens of MAIS complex (Mycobacterium avium-Mycobacterium intracellulare-M.scrofulaceum) were closely homologous even at the C-terminal regions which were variable among those of M. bovis BCG, Mycobacterium leprae and Mycobacterium kansasii.
UR - http://www.scopus.com/inward/record.url?scp=0027930111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027930111&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3083.1994.tb03446.x
DO - 10.1111/j.1365-3083.1994.tb03446.x
M3 - Article
C2 - 8047837
AN - SCOPUS:0027930111
VL - 40
SP - 165
EP - 170
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
SN - 0300-9475
IS - 2
ER -