Cloning, sequencing and expression in Escherichia coli of the gene for α antigen from Mycobacterium scrofulaceum

M. Takano, Naoya Oohara, A. Mizuno, T. Yamada

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The gene for the extracellular α antigen of Mycobacterium scrofulaceum (S-α) was cloned by using the α antigen gene fragments of Mycobacterium bovis BCG as probes. The complete nucleotide sequence was determined. The gene was expressed in Escherichia coil. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein. The deduced amino acid sequences were highly homologous to the a: antigen of the species of other mycobacteria. Interestingly, the a antigens of MAIS complex (Mycobacterium avium-Mycobacterium intracellulare-M.scrofulaceum) were closely homologous even at the C-terminal regions which were variable among those of M. bovis BCG, Mycobacterium leprae and Mycobacterium kansasii.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalScandinavian Journal of Immunology
Volume40
Issue number2
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Mycobacterium scrofulaceum
Mycobacterium bovis
Organism Cloning
Escherichia coli
Antigens
Mycobacterium
Amino Acids
Genes
Mycobacterium kansasii
Escherichia
Mycobacterium leprae
Mycobacterium avium Complex
Protein Sorting Signals
Amino Acid Sequence
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Cloning, sequencing and expression in Escherichia coli of the gene for α antigen from Mycobacterium scrofulaceum. / Takano, M.; Oohara, Naoya; Mizuno, A.; Yamada, T.

In: Scandinavian Journal of Immunology, Vol. 40, No. 2, 1994, p. 165-170.

Research output: Contribution to journalArticle

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