Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: Possible physiological roles of the calcitonin family in osmoregulation

Nobuo Suzuki, Toshio Sekiguchi, Honoo Satake, Kanoko Kato, Yudai Nishiyama, Hideya Takahashi, Janine A. Danks, T. John Martin, Atsuhiko Hattori, Masaki Nakano, Makiko Kakikawa, Sotoshi Yamada, Maho Ogoshi, Susumu Hyodo, Yoko Yamaguchi, Vishwajit S. Chowdhury, Kazuichi Hayakawa, Hisayuki Funahashi, Tatsuya Sakamoto, Yuichi Sasayama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin receptor (CTR) (46.1%), frog CTR (54.7%), and flounder CTR (48.9%). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5%, frog 50.7%, flounder 48.0%) and CTRs (human 43.2%, frog 49.1%, flounder 41.8%) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20% seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish.

Original languageEnglish
Pages (from-to)326-331
Number of pages6
JournalGene
Volume499
Issue number2
DOIs
Publication statusPublished - May 15 2012

Fingerprint

Calcitonin Receptors
Osmoregulation
Flounder
Calcitonin
Calcitonin Receptor-Like Protein
Organism Cloning
Anura
Amino Acid Receptors
Fishes
Seawater
Nervous System
Blood Vessels
Brain
Complementary DNA
Salt Gland
Kidney
Messenger RNA
Pituitary Gland
Open Reading Frames
Cartilage

Keywords

  • Calcitonin
  • Calcitonin gene-related peptide
  • Elasmobranch
  • Tissue expression

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei : Possible physiological roles of the calcitonin family in osmoregulation. / Suzuki, Nobuo; Sekiguchi, Toshio; Satake, Honoo; Kato, Kanoko; Nishiyama, Yudai; Takahashi, Hideya; Danks, Janine A.; Martin, T. John; Hattori, Atsuhiko; Nakano, Masaki; Kakikawa, Makiko; Yamada, Sotoshi; Ogoshi, Maho; Hyodo, Susumu; Yamaguchi, Yoko; Chowdhury, Vishwajit S.; Hayakawa, Kazuichi; Funahashi, Hisayuki; Sakamoto, Tatsuya; Sasayama, Yuichi.

In: Gene, Vol. 499, No. 2, 15.05.2012, p. 326-331.

Research output: Contribution to journalArticle

Suzuki, N, Sekiguchi, T, Satake, H, Kato, K, Nishiyama, Y, Takahashi, H, Danks, JA, Martin, TJ, Hattori, A, Nakano, M, Kakikawa, M, Yamada, S, Ogoshi, M, Hyodo, S, Yamaguchi, Y, Chowdhury, VS, Hayakawa, K, Funahashi, H, Sakamoto, T & Sasayama, Y 2012, 'Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: Possible physiological roles of the calcitonin family in osmoregulation', Gene, vol. 499, no. 2, pp. 326-331. https://doi.org/10.1016/j.gene.2012.03.042
Suzuki, Nobuo ; Sekiguchi, Toshio ; Satake, Honoo ; Kato, Kanoko ; Nishiyama, Yudai ; Takahashi, Hideya ; Danks, Janine A. ; Martin, T. John ; Hattori, Atsuhiko ; Nakano, Masaki ; Kakikawa, Makiko ; Yamada, Sotoshi ; Ogoshi, Maho ; Hyodo, Susumu ; Yamaguchi, Yoko ; Chowdhury, Vishwajit S. ; Hayakawa, Kazuichi ; Funahashi, Hisayuki ; Sakamoto, Tatsuya ; Sasayama, Yuichi. / Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei : Possible physiological roles of the calcitonin family in osmoregulation. In: Gene. 2012 ; Vol. 499, No. 2. pp. 326-331.
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abstract = "In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5{\%}, frog CRLR is 64.7{\%}, and flounder CRLR is 61.2{\%} and this was higher than to human calcitonin receptor (CTR) (46.1{\%}), frog CTR (54.7{\%}), and flounder CTR (48.9{\%}). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5{\%}, frog 50.7{\%}, flounder 48.0{\%}) and CTRs (human 43.2{\%}, frog 49.1{\%}, flounder 41.8{\%}) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20{\%} seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish.",
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T1 - Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei

T2 - Possible physiological roles of the calcitonin family in osmoregulation

AU - Suzuki, Nobuo

AU - Sekiguchi, Toshio

AU - Satake, Honoo

AU - Kato, Kanoko

AU - Nishiyama, Yudai

AU - Takahashi, Hideya

AU - Danks, Janine A.

AU - Martin, T. John

AU - Hattori, Atsuhiko

AU - Nakano, Masaki

AU - Kakikawa, Makiko

AU - Yamada, Sotoshi

AU - Ogoshi, Maho

AU - Hyodo, Susumu

AU - Yamaguchi, Yoko

AU - Chowdhury, Vishwajit S.

AU - Hayakawa, Kazuichi

AU - Funahashi, Hisayuki

AU - Sakamoto, Tatsuya

AU - Sasayama, Yuichi

PY - 2012/5/15

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N2 - In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin receptor (CTR) (46.1%), frog CTR (54.7%), and flounder CTR (48.9%). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5%, frog 50.7%, flounder 48.0%) and CTRs (human 43.2%, frog 49.1%, flounder 41.8%) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20% seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish.

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