Cloning of citrate permease gene of Lactococcus lactis subsp. Lactis biovar diacetylactis NIAI N-7 and expression in citrate-negative lactococci

Hidetoshi Morita, K. Kamizono, S. Nakamura, Y. Fujita, R. Sakata, Y. Nagata, L. L. McKay

Research output: Contribution to journalArticle

Abstract

The putative citrate permease (Cit-P) plasmid DNA (pN7CP) from Lactococcus lactis subsp. lactis biovar diacetylactis (L diacetylactis) NIAI N-7 was cloned. Cit- P+ transformants possessed pIL253 with a 3.8-kb fragment (pKAK1) or a 2.0-kb fragment (pKAK7). No Xba I site was present in the 3.8-kb of Xba I-Xba I fragment from pN7CP. It may thus be possible to obtain the 2.0-kb fragment by deleting the 3.8-kb fragment. Assessment was made of citrate-transporting activity in transformants KAK1 and KAK7. Several Cit- lactococci were transformed by pKAK7. Although all their transformants except KK-7LM and KK-7SL of Lactococcus lactis subsp. lactis (L. lactis) LM0230 and NIAI SLN were incapable of transporting citrate, transformants KK-7LM and KK- 7SL showed the Cit+ phenotype and citrate-transporting activity. L. diacetylactis and L. lactis were classified under citrate metabolism. However, the present findings strongly indicate some strains of L. lactis to quite likely be the original citrate utilizers but simply to have lost the Cit-P gene.

Original languageEnglish
Pages (from-to)138-141
Number of pages4
JournalMilchwissenschaft
Volume52
Issue number3
Publication statusPublished - 1997
Externally publishedYes

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Lactococcus lactis subsp. lactis bv. diacetylactis
Lactococcus
Citric Acid
citrates
Lactococcus lactis
Organism Cloning
molecular cloning
Lactococcus lactis subsp. lactis
Genes
genes
Membrane Transport Proteins
Lactococcus lactis citP protein
plasmids
Plasmids
Phenotype
phenotype
metabolism

ASJC Scopus subject areas

  • Food Science

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Cloning of citrate permease gene of Lactococcus lactis subsp. Lactis biovar diacetylactis NIAI N-7 and expression in citrate-negative lactococci. / Morita, Hidetoshi; Kamizono, K.; Nakamura, S.; Fujita, Y.; Sakata, R.; Nagata, Y.; McKay, L. L.

In: Milchwissenschaft, Vol. 52, No. 3, 1997, p. 138-141.

Research output: Contribution to journalArticle

Morita, Hidetoshi ; Kamizono, K. ; Nakamura, S. ; Fujita, Y. ; Sakata, R. ; Nagata, Y. ; McKay, L. L. / Cloning of citrate permease gene of Lactococcus lactis subsp. Lactis biovar diacetylactis NIAI N-7 and expression in citrate-negative lactococci. In: Milchwissenschaft. 1997 ; Vol. 52, No. 3. pp. 138-141.
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T1 - Cloning of citrate permease gene of Lactococcus lactis subsp. Lactis biovar diacetylactis NIAI N-7 and expression in citrate-negative lactococci

AU - Morita, Hidetoshi

AU - Kamizono, K.

AU - Nakamura, S.

AU - Fujita, Y.

AU - Sakata, R.

AU - Nagata, Y.

AU - McKay, L. L.

PY - 1997

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