Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family

Minami Matsui, Shigemi Sasamoto, Tetsuo Kunieda, Nobuo Nomura, Ryotaro Ishizaki

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalGene
Volume76
Issue number2
DOIs
Publication statusPublished - Mar 30 1989
Externally publishedYes

Keywords

  • Recombinant DNA
  • genomic library
  • homopyrimidine-homopurine tract
  • phage λ vector
  • plant gene

ASJC Scopus subject areas

  • Genetics

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