TY - JOUR
T1 - Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family
AU - Matsui, Minami
AU - Sasamoto, Shigemi
AU - Kunieda, Tetsuo
AU - Nomura, Nobuo
AU - Ishizaki, Ryotaro
N1 - Funding Information:
We thank E.M. Meyerowitz for a genomic library ofA. thaliana and N. Goto for seedso f the wild type. We are also grateful to N. Miyajima, A. Ito and M. Yamamoto for help in computer analysis and the Japanese Cancer Research Resources Bank for an oncogenep robe. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1989/3/30
Y1 - 1989/3/30
N2 - A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.
AB - A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.
KW - Recombinant DNA
KW - genomic library
KW - homopyrimidine-homopurine tract
KW - phage λ vector
KW - plant gene
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U2 - 10.1016/0378-1119(89)90171-6
DO - 10.1016/0378-1119(89)90171-6
M3 - Article
C2 - 2666259
AN - SCOPUS:0024578092
VL - 76
SP - 313
EP - 319
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -