Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family

Minami Matsui; Shigemi Sasamoto; Tetsuo Kunieda; Nobuo Nomura; Ryotaro Ishizaki

doi:10.1016/0378-1119(89)90171-6

Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family

Minami Matsui, Shigemi Sasamoto, Tetsuo Kunieda, Nobuo Nomura, Ryotaro Ishizaki

Research output: Contribution to journal › Article

69 Citations (Scopus)

Abstract

A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (M_r 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- A_C^AAAAACCAAAGTG_T^AAT_C^G-3′, was found three times in the 5'-noncoding region and once in the intron.

Original language	English
Pages (from-to)	313-319
Number of pages	7
Journal	Gene
Volume	76
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(89)90171-6
Publication status	Published - Mar 30 1989
Externally published	Yes

Keywords

Recombinant DNA
genomic library
homopyrimidine-homopurine tract
phage λ vector
plant gene

ASJC Scopus subject areas

Genetics

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Matsui, M., Sasamoto, S., Kunieda, T., Nomura, N., & Ishizaki, R. (1989). Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family. Gene, 76(2), 313-319. https://doi.org/10.1016/0378-1119(89)90171-6

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abstract = "A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.",

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AU - Matsui, Minami

AU - Sasamoto, Shigemi

AU - Kunieda, Tetsuo

AU - Nomura, Nobuo

AU - Ishizaki, Ryotaro

PY - 1989/3/30

Y1 - 1989/3/30

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KW - homopyrimidine-homopurine tract

KW - phage λ vector

KW - plant gene

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