Abstract
A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.
| Original language | English |
|---|---|
| Pages (from-to) | 313-319 |
| Number of pages | 7 |
| Journal | Gene |
| Volume | 76 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 30 1989 |
| Externally published | Yes |
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Keywords
- genomic library
- homopyrimidine-homopurine tract
- phage λ vector
- plant gene
- Recombinant DNA
ASJC Scopus subject areas
- Genetics
Cite this
Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family. / Matsui, Minami; Sasamoto, Shigemi; Kunieda, Tetsuo; Nomura, Nobuo; Ishizaki, Ryotaro.
In: Gene, Vol. 76, No. 2, 30.03.1989, p. 313-319.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family
AU - Matsui, Minami
AU - Sasamoto, Shigemi
AU - Kunieda, Tetsuo
AU - Nomura, Nobuo
AU - Ishizaki, Ryotaro
PY - 1989/3/30
Y1 - 1989/3/30
N2 - A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.
AB - A putative Arabidopsis thaliana gene, ara, homologous to the ras-related gene family was isolated from a genomic library by low-stringency hybridization with a v-Ha-ras probe. The putative gene has a coding frame for a 218-residue protein (Mr 24210), which is interrupted by one intron. The amino acid residues conserved in proteins of the ras gene family, which are important for GTP binding, GTP hydrolysis and membrane attachment, are also present in the ara protein. An unusually long homopyrimidine-homopurine tract was found about 100 bp upstream from the methionine start codon. A unique sequence, 5′- ACAAAAACCAAAGTGTAATCG-3′, was found three times in the 5'-noncoding region and once in the intron.
KW - genomic library
KW - homopyrimidine-homopurine tract
KW - phage λ vector
KW - plant gene
KW - Recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=0024578092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024578092&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(89)90171-6
DO - 10.1016/0378-1119(89)90171-6
M3 - Article
VL - 76
SP - 313
EP - 319
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -