Cloning of a novel amphiphysin isoform edna composed of multiple splicing variants and their differential tissue expression

Kimiko Tsutsui, Yukihide Maeda, Ken Tsutsui, Kuniaki Sa, Shuji Seki, Akira Tokunaga

Research output: Contribution to journalArticle

Abstract

Amphiphysin, an Stt3-domain containing protein concentrated in nerve terminals, is believed to be involved in the synaptic vesicle recycling. We have cloned cDNAs of a novel isoform of amphiphysin from mammals (man, rat, and mouse) by utilizing sequence information for homologous ESTs accumulated in databases. More than 10 subtypes of the isoform with 50-60 amino acid identity to the human amphiphysin were identified by a conventional library screening and PCR amplification of cDNA libraries. Each subtype probably represents a splicing variant of a single gene transcript. Analysis of mRNA expression in various tissues by RT-PCR showed that the isoform is more ubiquitously distributed than the canonical amphiphysin which is almost restricted to the CNS. Expression spectrum of the isoform subtypes, however, is significantly different in several tissues examined, suggesting that they are involved in endocytotic processes that are unique to each cell type.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997

Fingerprint

Cloning
Organism Cloning
molecular cloning
Protein Isoforms
Tissue
cDNA libraries
recycling
nerve tissue
reverse transcriptase polymerase chain reaction
mammals
screening
Polymerase Chain Reaction
amino acids
Mammals
Synaptic Vesicles
Expressed Sequence Tags
rats
mice
Sequence Homology
Gene Library

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Cloning of a novel amphiphysin isoform edna composed of multiple splicing variants and their differential tissue expression. / Tsutsui, Kimiko; Maeda, Yukihide; Tsutsui, Ken; Sa, Kuniaki; Seki, Shuji; Tokunaga, Akira.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Tsutsui, Kimiko ; Maeda, Yukihide ; Tsutsui, Ken ; Sa, Kuniaki ; Seki, Shuji ; Tokunaga, Akira. / Cloning of a novel amphiphysin isoform edna composed of multiple splicing variants and their differential tissue expression. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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AU - Tokunaga, Akira

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