Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA

Tohru Nakanishi, Yusuke Kimura, Tomoo Tamura, Hiroyuki Ichikawa, Yu Ichiro Yamaai, Tomosada Sugimoto, Masaharu Takigawa

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.

Original languageEnglish
Pages (from-to)206-210
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume234
Issue number1
DOIs
Publication statusPublished - May 8 1997

Fingerprint

Connective Tissue Growth Factor
Cloning
Chondrocytes
Organism Cloning
Display devices
Cells
Cell Line
Polymerase Chain Reaction
Messenger RNA
Cartilage
Nucleotides
Cell Culture Techniques
Chondrosarcoma
Osteogenesis
Northern Blotting
In Situ Hybridization
Spine
Rabbits
DNA
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA. / Nakanishi, Tohru; Kimura, Yusuke; Tamura, Tomoo; Ichikawa, Hiroyuki; Yamaai, Yu Ichiro; Sugimoto, Tomosada; Takigawa, Masaharu.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 1, 08.05.1997, p. 206-210.

Research output: Contribution to journalArticle

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