Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA

Tohru Nakanishi; Yusuke Kimura; Tomoo Tamura; Hiroyuki Ichikawa; Yuichiro Yamaai; Tomosada Sugimoto; Masaharu Takigawa

doi:10.1006/bbrc.1997.6528

Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA

Tohru Nakanishi, Yusuke Kimura, Tomoo Tamura, Hiroyuki Ichikawa, Yuichiro Yamaai, Tomosada Sugimoto, Masaharu Takigawa

Dental School

Research output: Contribution to journal › Article › peer-review

172 Citations (Scopus)

Abstract

Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.

Original language	English
Pages (from-to)	206-210
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	234
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.6528
Publication status	Published - May 8 1997

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1997.6528

Cite this

Nakanishi, T., Kimura, Y., Tamura, T., Ichikawa, H., Yamaai, Y., Sugimoto, T., & Takigawa, M. (1997). Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA. Biochemical and Biophysical Research Communications, 234(1), 206-210. https://doi.org/10.1006/bbrc.1997.6528

Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA. / Nakanishi, Tohru; Kimura, Yusuke; Tamura, Tomoo et al.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 1, 08.05.1997, p. 206-210.

Research output: Contribution to journal › Article › peer-review

Nakanishi, T, Kimura, Y, Tamura, T, Ichikawa, H, Yamaai, Y, Sugimoto, T & Takigawa, M 1997, 'Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA', Biochemical and Biophysical Research Communications, vol. 234, no. 1, pp. 206-210. https://doi.org/10.1006/bbrc.1997.6528

Nakanishi T, Kimura Y, Tamura T, Ichikawa H, Yamaai Y, Sugimoto T et al. Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA. Biochemical and Biophysical Research Communications. 1997 May 8;234(1):206-210. doi: 10.1006/bbrc.1997.6528

@article{5a37d04f99cc403db431d07492c37f00,

title = "Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA",

abstract = "Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.",

author = "Tohru Nakanishi and Yusuke Kimura and Tomoo Tamura and Hiroyuki Ichikawa and Yuichiro Yamaai and Tomosada Sugimoto and Masaharu Takigawa",

note = "Funding Information: We thank Dr. Akira Yamaguchi (Showa University Dental School) for providing Saos-2. We also thank Dr. Kojiro Takahashi and Miss Takako Hattori for useful discussion and Mrs. Etsuko Fujisawa for secretarial assistance. This work was supported in part by Grants-in-Aid for Scienti{\textregistered}c Research from the Ministry of Education, Science, Sports, and Culture of Japan (M.T.) and grants from the Yamada Science Foundation (M.T.), the Sanyo Hohso Foundation (M.T.), the Sagawa Cancer Research Foundation (M.T.), and the Naito Foundation (M.T.), and by funds from Hoechst Japan.",

year = "1997",

month = may,

day = "8",

doi = "10.1006/bbrc.1997.6528",

language = "English",

volume = "234",

pages = "206--210",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA

AU - Nakanishi, Tohru

AU - Kimura, Yusuke

AU - Tamura, Tomoo

AU - Ichikawa, Hiroyuki

AU - Yamaai, Yuichiro

AU - Sugimoto, Tomosada

AU - Takigawa, Masaharu

N1 - Funding Information: We thank Dr. Akira Yamaguchi (Showa University Dental School) for providing Saos-2. We also thank Dr. Kojiro Takahashi and Miss Takako Hattori for useful discussion and Mrs. Etsuko Fujisawa for secretarial assistance. This work was supported in part by Grants-in-Aid for Scienti®c Research from the Ministry of Education, Science, Sports, and Culture of Japan (M.T.) and grants from the Yamada Science Foundation (M.T.), the Sanyo Hohso Foundation (M.T.), the Sagawa Cancer Research Foundation (M.T.), and the Naito Foundation (M.T.), and by funds from Hoechst Japan.

PY - 1997/5/8

Y1 - 1997/5/8

N2 - Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.

AB - Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Northern blot analysis showed that CTGF was highly expressed in HCS cells and rabbit growth cartilage cells in culture but was not expressed in osteoblastic cells in culture. In situ hybridization revealed that CTGF was expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. The expression of CTGF in HCS cells was up-regulated by the addition of TGF-β or BMP-2. These findings suggest that CTGF participates in endochondral ossification.

UR - http://www.scopus.com/inward/record.url?scp=0030907579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030907579&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.6528

DO - 10.1006/bbrc.1997.6528

M3 - Article

C2 - 9168990

AN - SCOPUS:0030907579

SN - 0006-291X

VL - 234

SP - 206

EP - 210

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this