Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene

Jonatan Montpetit, Julien Vivancos, Namiki Mitani, Naoki Yamaji, Wilfried Rémus-Borel, François Belzile, Jian Feng Ma, Richard R. Bélanger

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Silicon (Si) is known to be beneficial to plants, namely in alleviating biotic and abiotic stresses. The magnitude of such positive effects is associated with a plant's natural ability to absorb Si. Many grasses can accumulate as much as 10% on a dry weight basis while most dicots, including Arabidopsis, will accumulate less than 0.1%. In this report, we describe the cloning and functional characterization of TaLsi1, a wheat Si transporter gene. In addition, we developed a heterologous system for the study of Si uptake in plants by introducing TaLsi1 and OsLsi1, its ortholog in rice, into Arabidopsis, a species with a very low innate Si uptake capacity. When expressed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed in cells of roots and shoots. Such constitutive expression of TaLsi1 or OsLsi1 resulted in a fourfold to fivefold increase in Si accumulation in transformed plants compared to WT. However, this Si absorption caused deleterious symptoms. When the wheat transporter was expressed under the control of a root-specific promoter (a boron transporter gene (AtNIP5;1) promoter), a similar increase in Si absorption was noted but the plants did not exhibit symptoms and grew normally. These results demonstrate that TaLsi1 is indeed a functional Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies Si transporter as its expression in Arabidopsis leads to increased Si uptake, but that this expression must be confined to root cells for healthy plant development. The availability of this heterologous expression system will facilitate further studies into the mechanisms and benefits of Si uptake.

Original languageEnglish
Pages (from-to)35-46
Number of pages12
JournalPlant Molecular Biology
Volume79
Issue number1-2
DOIs
Publication statusPublished - May 2012

Fingerprint

Silicon
silicon
Triticum
transporters
Organism Cloning
molecular cloning
wheat
Genes
genes
Arabidopsis
uptake mechanisms
Plant Development
promoter regions
signs and symptoms (plants)
plant development
Boron
cells
biotic stress
Poaceae
Magnoliopsida

Keywords

  • Aquaporins
  • Lsi1 genes
  • Silicon transport
  • Transgenic Arabidopsis
  • Wheat

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

Cite this

Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene. / Montpetit, Jonatan; Vivancos, Julien; Mitani, Namiki; Yamaji, Naoki; Rémus-Borel, Wilfried; Belzile, François; Ma, Jian Feng; Bélanger, Richard R.

In: Plant Molecular Biology, Vol. 79, No. 1-2, 05.2012, p. 35-46.

Research output: Contribution to journalArticle

Montpetit, Jonatan ; Vivancos, Julien ; Mitani, Namiki ; Yamaji, Naoki ; Rémus-Borel, Wilfried ; Belzile, François ; Ma, Jian Feng ; Bélanger, Richard R. / Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene. In: Plant Molecular Biology. 2012 ; Vol. 79, No. 1-2. pp. 35-46.
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AU - Montpetit, Jonatan

AU - Vivancos, Julien

AU - Mitani, Namiki

AU - Yamaji, Naoki

AU - Rémus-Borel, Wilfried

AU - Belzile, François

AU - Ma, Jian Feng

AU - Bélanger, Richard R.

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