Cloning, characterization, and expression of xyloglucan endotransglucosylase/hydrolase and expansin genes associated with petal growth and development during carnation flower opening

Taro Harada, Yuka Torii, Shigeto Morita, Reiko Onodera, Yoshinao Hara, Ryusuke Yokoyama, Kazuhiko Nishitani, Shigeru Satoh

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Growth of petal cells is a basis for expansion and morphogenesis (outward bending) of petals during opening of carnation flowers (Dianthus caryophyllus L.). Petal growth progressed through elongation in the early stage, expansion with outward bending in the middle stage, and expansion of the whole area in the late stage of flower opening. In the present study, four cDNAs encoding xyloglucan endotransglucosylase/hydrolase (XTH) (DcXTH1-DcXTH4) and three cDNAs encoding expansin (DcEXPA1-DcEXPA3) were cloned from petals of opening carnation flowers and characterized. Real-time reverse transcription-PCR analyses showed that transcript levels of XTH and expansin genes accumulated differently in floral and vegetative tissues of carnation plants with opening flowers, indicating regulated expression of these genes. DcXTH2 and DcXTH3 transcripts were detected in large quantities in petals as compared with other tissues. DcEXPA1 and DcEXPA2 transcripts were markedly accumulated in petals of opening flowers. The action of XTH in growing petal tissues was confirmed by in situ staining of xyloglucan endotransglucosylase (XET) activity using a rhodamine-labelled xyloglucan nonasaccharide as a substrate. Based on the present findings, it is suggested that two XTH genes (DcXTH2 and DcXTH3) and two expansin genes (DcEXPA1 and DcEXPA2) are associated with petal growth and development during carnation flower opening.

Original languageEnglish
Pages (from-to)815-823
Number of pages9
JournalJournal of Experimental Botany
Volume62
Issue number2
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

xyloglucan - xyloglucosyltransferase
Dianthus
xyloglucan:xyloglucosyl transferase
expansins
Dianthus caryophyllus
hydrolases
Growth and Development
corolla
Organism Cloning
molecular cloning
growth and development
flowers
Genes
genes
Complementary DNA
Rhodamines
Growth
Morphogenesis
xyloglucans
Reverse Transcription

Keywords

  • Carnation
  • expansin
  • flower opening
  • gene expression
  • in situ XET staining
  • petal cell growth
  • xyloglucan endotransglycosylase/hydrolase (XTH)

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Cloning, characterization, and expression of xyloglucan endotransglucosylase/hydrolase and expansin genes associated with petal growth and development during carnation flower opening. / Harada, Taro; Torii, Yuka; Morita, Shigeto; Onodera, Reiko; Hara, Yoshinao; Yokoyama, Ryusuke; Nishitani, Kazuhiko; Satoh, Shigeru.

In: Journal of Experimental Botany, Vol. 62, No. 2, 01.2011, p. 815-823.

Research output: Contribution to journalArticle

Harada, Taro ; Torii, Yuka ; Morita, Shigeto ; Onodera, Reiko ; Hara, Yoshinao ; Yokoyama, Ryusuke ; Nishitani, Kazuhiko ; Satoh, Shigeru. / Cloning, characterization, and expression of xyloglucan endotransglucosylase/hydrolase and expansin genes associated with petal growth and development during carnation flower opening. In: Journal of Experimental Botany. 2011 ; Vol. 62, No. 2. pp. 815-823.
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