Cloning and sequencing of the meIB gene encoding the melibiose permease of Salmonella typhimurium LT2

Kaori Mizushima, Shuji Awakihara, Masayuki Kuroda, Tetsuya Ishikawa, Masaaki Tsuda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The nucleotide sequence of the melB gene coding for the Na+(Li+)/melibiose symporter of Salmonella typhimurium LT2 was determined, and its amino acid sequence was deduced. It consists of 1428 bp, corresponding to a protein of 476 amino acid residues (calculated molecular weight 52800). The amino acid sequence is homologous to that of the melibiose permease of Escherichia coli K12, with 85% identical residues. All, except one, of the amino acid residues that have been reported to be important for cation or substrate recognition in the melibiose permease of E. coli are conserved in the melibiose permease of S. typhimurium. In addition, part of the sequence resembles the lactose permease of Streptococcus thermophilus, the animal glucose transporter (GLUT1), the plasmid-coded raffinose permease (RafB), and the NADH-ubiquinone oxidoreductase chain 4 (Nuo4) of Aspergillus amstelodami.

Original languageEnglish
Pages (from-to)74-80
Number of pages7
JournalMGG Molecular & General Genetics
Volume234
Issue number1
DOIs
Publication statusPublished - Jul 1 1992

Keywords

  • Functional domains
  • Gene melB
  • Melibiose permease
  • Salmonella typhimurium
  • Sequence

ASJC Scopus subject areas

  • Genetics

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