Cloning and sequencing of the gene for Na+/H+ antiporter of vibrio parahaemolyticus

Kaori Nozaki, Kei Inaba, Teruo Kuroda, Masaaki Tsuda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A gene encoding an Na+/H+ antiporter was cloned from Vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the presence of 0.2 M NaCl (or 10 mM LiCl). These cells were originally unable to grow under such conditions because of the lack of major Na+(Li+)/H+ antiporters. We detected Na+/H+ antiporter activity due to the gene in membrane vesicles. The gene was sequenced and the deduced amino acid sequence was found to be 72% identical to the NhaB Na+/H+ antiporter of E. coli.

Original languageEnglish
Pages (from-to)774-779
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume222
Issue number3
DOIs
Publication statusPublished - May 24 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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