Cloning and sequencing of the gene for Na+/H+ antiporter of vibrio parahaemolyticus

Kaori Nozaki, Kei Inaba, Teruo Kuroda, Masaaki Tsuda, Tomofusa Tsuchiya

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Abstract

A gene encoding an Na+/H+ antiporter was cloned from Vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the presence of 0.2 M NaCl (or 10 mM LiCl). These cells were originally unable to grow under such conditions because of the lack of major Na+(Li+)/H+ antiporters. We detected Na+/H+ antiporter activity due to the gene in membrane vesicles. The gene was sequenced and the deduced amino acid sequence was found to be 72% identical to the NhaB Na+/H+ antiporter of E. coli.

Original languageEnglish
Pages (from-to)774-779
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume222
Issue number3
DOIs
Publication statusPublished - May 24 1996

Fingerprint

Vibrio parahaemolyticus
Sodium-Hydrogen Antiporter
Cloning
Escherichia coli
Organism Cloning
Genes
Antiporters
Gene encoding
Plasmids
Membranes
Amino Acids
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and sequencing of the gene for Na+/H+ antiporter of vibrio parahaemolyticus. / Nozaki, Kaori; Inaba, Kei; Kuroda, Teruo; Tsuda, Masaaki; Tsuchiya, Tomofusa.

In: Biochemical and Biophysical Research Communications, Vol. 222, No. 3, 24.05.1996, p. 774-779.

Research output: Contribution to journalArticle

Nozaki, Kaori ; Inaba, Kei ; Kuroda, Teruo ; Tsuda, Masaaki ; Tsuchiya, Tomofusa. / Cloning and sequencing of the gene for Na+/H+ antiporter of vibrio parahaemolyticus. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 222, No. 3. pp. 774-779.
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