Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes

Naoya Oohara, K. Matsuo, R. Yamaguchi, A. Yamazaki, H. Tasaka, T. Yamada

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The complete nucleotide sequence of α antigen secreted from Mycobacterium avium (A-α) was determined. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein with a molecular mass of 30,811 Da. This is the first sequence of A-α. Comparisons between A-α and α antigens of Mycobacterium leprae, Mycobacterium bovis BCG, and Mycobacterium kansasii showed highly homologous regions which suggested a conserved functional domain and two less-homologous regions. Serological analysis of recombinant A-α, expressed by a series of deletion constructs, indicated the possibility that A-α carries at least six B-cell epitopes. The three antigenic determinants were common to Mycobacterium tuberculosis, M. kansasii, and M. avium. The results also suggested the possibility that there are three species-specific epitopes.

Original languageEnglish
Pages (from-to)1173-1179
Number of pages7
JournalInfection and Immunity
Volume61
Issue number4
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

B-Lymphocyte Epitopes
Mycobacterium avium
Mycobacterium kansasii
Organism Cloning
Mycobacterium bovis
Antigens
Amino Acids
Epitopes
Genes
Mycobacterium leprae
Protein Sorting Signals
Mycobacterium tuberculosis
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Oohara, N., Matsuo, K., Yamaguchi, R., Yamazaki, A., Tasaka, H., & Yamada, T. (1993). Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes. Infection and Immunity, 61(4), 1173-1179.

Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes. / Oohara, Naoya; Matsuo, K.; Yamaguchi, R.; Yamazaki, A.; Tasaka, H.; Yamada, T.

In: Infection and Immunity, Vol. 61, No. 4, 1993, p. 1173-1179.

Research output: Contribution to journalArticle

Oohara, N, Matsuo, K, Yamaguchi, R, Yamazaki, A, Tasaka, H & Yamada, T 1993, 'Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes', Infection and Immunity, vol. 61, no. 4, pp. 1173-1179.
Oohara, Naoya ; Matsuo, K. ; Yamaguchi, R. ; Yamazaki, A. ; Tasaka, H. ; Yamada, T. / Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes. In: Infection and Immunity. 1993 ; Vol. 61, No. 4. pp. 1173-1179.
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