Abstract
The complete nucleotide sequence of α antigen secreted from Mycobacterium avium (A-α) was determined. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein with a molecular mass of 30,811 Da. This is the first sequence of A-α. Comparisons between A-α and α antigens of Mycobacterium leprae, Mycobacterium bovis BCG, and Mycobacterium kansasii showed highly homologous regions which suggested a conserved functional domain and two less-homologous regions. Serological analysis of recombinant A-α, expressed by a series of deletion constructs, indicated the possibility that A-α carries at least six B-cell epitopes. The three antigenic determinants were common to Mycobacterium tuberculosis, M. kansasii, and M. avium. The results also suggested the possibility that there are three species-specific epitopes.
| Original language | English |
|---|---|
| Pages (from-to) | 1173-1179 |
| Number of pages | 7 |
| Journal | Infection and Immunity |
| Volume | 61 |
| Issue number | 4 |
| Publication status | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes. / Oohara, Naoya; Matsuo, K.; Yamaguchi, R.; Yamazaki, A.; Tasaka, H.; Yamada, T.
In: Infection and Immunity, Vol. 61, No. 4, 1993, p. 1173-1179.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes
AU - Oohara, Naoya
AU - Matsuo, K.
AU - Yamaguchi, R.
AU - Yamazaki, A.
AU - Tasaka, H.
AU - Yamada, T.
PY - 1993
Y1 - 1993
N2 - The complete nucleotide sequence of α antigen secreted from Mycobacterium avium (A-α) was determined. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein with a molecular mass of 30,811 Da. This is the first sequence of A-α. Comparisons between A-α and α antigens of Mycobacterium leprae, Mycobacterium bovis BCG, and Mycobacterium kansasii showed highly homologous regions which suggested a conserved functional domain and two less-homologous regions. Serological analysis of recombinant A-α, expressed by a series of deletion constructs, indicated the possibility that A-α carries at least six B-cell epitopes. The three antigenic determinants were common to Mycobacterium tuberculosis, M. kansasii, and M. avium. The results also suggested the possibility that there are three species-specific epitopes.
AB - The complete nucleotide sequence of α antigen secreted from Mycobacterium avium (A-α) was determined. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein with a molecular mass of 30,811 Da. This is the first sequence of A-α. Comparisons between A-α and α antigens of Mycobacterium leprae, Mycobacterium bovis BCG, and Mycobacterium kansasii showed highly homologous regions which suggested a conserved functional domain and two less-homologous regions. Serological analysis of recombinant A-α, expressed by a series of deletion constructs, indicated the possibility that A-α carries at least six B-cell epitopes. The three antigenic determinants were common to Mycobacterium tuberculosis, M. kansasii, and M. avium. The results also suggested the possibility that there are three species-specific epitopes.
UR - http://www.scopus.com/inward/record.url?scp=0027415633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027415633&partnerID=8YFLogxK
M3 - Article
C2 - 7681039
AN - SCOPUS:0027415633
VL - 61
SP - 1173
EP - 1179
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 4
ER -