TY - JOUR
T1 - Cloning and sequencing of the gene for α antigen from Mycobacterium avium and mapping of B-cell epitopes
AU - Ohara, N.
AU - Matsuo, K.
AU - Yamaguchi, R.
AU - Yamazaki, A.
AU - Tasaka, H.
AU - Yamada, T.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - The complete nucleotide sequence of α antigen secreted from Mycobacterium avium (A-α) was determined. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein with a molecular mass of 30,811 Da. This is the first sequence of A-α. Comparisons between A-α and α antigens of Mycobacterium leprae, Mycobacterium bovis BCG, and Mycobacterium kansasii showed highly homologous regions which suggested a conserved functional domain and two less-homologous regions. Serological analysis of recombinant A-α, expressed by a series of deletion constructs, indicated the possibility that A-α carries at least six B-cell epitopes. The three antigenic determinants were common to Mycobacterium tuberculosis, M. kansasii, and M. avium. The results also suggested the possibility that there are three species-specific epitopes.
AB - The complete nucleotide sequence of α antigen secreted from Mycobacterium avium (A-α) was determined. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein with a molecular mass of 30,811 Da. This is the first sequence of A-α. Comparisons between A-α and α antigens of Mycobacterium leprae, Mycobacterium bovis BCG, and Mycobacterium kansasii showed highly homologous regions which suggested a conserved functional domain and two less-homologous regions. Serological analysis of recombinant A-α, expressed by a series of deletion constructs, indicated the possibility that A-α carries at least six B-cell epitopes. The three antigenic determinants were common to Mycobacterium tuberculosis, M. kansasii, and M. avium. The results also suggested the possibility that there are three species-specific epitopes.
UR - http://www.scopus.com/inward/record.url?scp=0027415633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027415633&partnerID=8YFLogxK
U2 - 10.1128/iai.61.4.1173-1179.1993
DO - 10.1128/iai.61.4.1173-1179.1993
M3 - Article
C2 - 7681039
AN - SCOPUS:0027415633
VL - 61
SP - 1173
EP - 1179
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 4
ER -