Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

H. Hayashida; H. Hotokezaka; N. Ohara; M. Kimura; O. Takagi; T. Yamada

doi:10.1111/j.1399-302X.1997.tb00375.x

Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

H. Hayashida, H. Hotokezaka, N. Ohara, M. Kimura, O. Takagi, T. Yamada

Research output: Contribution to journal › Article › peer-review

Abstract

We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

Original language	English
Pages (from-to)	174-177
Number of pages	4
Journal	Oral microbiology and immunology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1111/j.1399-302X.1997.tb00375.x
Publication status	Published - Jun 1997
Externally published	Yes

Keywords

Actinobacillus actinomycetemcomitans
Cloning
S10 operon

ASJC Scopus subject areas

Microbiology
Immunology
Dentistry(all)
Microbiology (medical)

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10.1111/j.1399-302X.1997.tb00375.x

Cite this

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title = "Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4",

abstract = "We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.",

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T1 - Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

AU - Hayashida, H.

AU - Hotokezaka, H.

AU - Ohara, N.

AU - Kimura, M.

AU - Takagi, O.

AU - Yamada, T.

PY - 1997/6

Y1 - 1997/6

N2 - We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

AB - We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

KW - Actinobacillus actinomycetemcomitans

KW - Cloning

KW - S10 operon

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Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

Abstract

Keywords

ASJC Scopus subject areas

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