Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

H. Hayashida; H. Hotokezaka; N. Ohara; M. Kimura; O. Takagi; T. Yamada

doi:10.1111/j.1399-302X.1997.tb00375.x

Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

H. Hayashida, H. Hotokezaka, N. Ohara, M. Kimura, O. Takagi, T. Yamada

Research output: Contribution to journal › Article › peer-review

Abstract

We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

Original language	English
Pages (from-to)	174-177
Number of pages	4
Journal	Oral microbiology and immunology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1111/j.1399-302X.1997.tb00375.x
Publication status	Published - Jun 1997
Externally published	Yes

Keywords

Actinobacillus actinomycetemcomitans
Cloning
S10 operon

ASJC Scopus subject areas

Microbiology
Immunology
Dentistry(all)
Microbiology (medical)

Access to Document

10.1111/j.1399-302X.1997.tb00375.x

Fingerprint Dive into the research topics of 'Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4'. Together they form a unique fingerprint.

Cite this

@article{1f26023c4eec4da2b37667b8a8ec45a0,

title = "Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4",

abstract = "We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.",

keywords = "Actinobacillus actinomycetemcomitans, Cloning, S10 operon",

author = "H. Hayashida and H. Hotokezaka and N. Ohara and M. Kimura and O. Takagi and T. Yamada",

year = "1997",

month = jun,

doi = "10.1111/j.1399-302X.1997.tb00375.x",

language = "English",

volume = "12",

pages = "174--177",

journal = "Molecular Oral Microbiology",

issn = "2041-1006",

publisher = "American Journal of Nursing Company",

number = "3",

}

TY - JOUR

T1 - Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4

AU - Hayashida, H.

AU - Hotokezaka, H.

AU - Ohara, N.

AU - Kimura, M.

AU - Takagi, O.

AU - Yamada, T.

PY - 1997/6

Y1 - 1997/6

N2 - We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

AB - We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3% 100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

KW - Actinobacillus actinomycetemcomitans

KW - Cloning

KW - S10 operon

UR - http://www.scopus.com/inward/record.url?scp=0031170995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031170995&partnerID=8YFLogxK

U2 - 10.1111/j.1399-302X.1997.tb00375.x

DO - 10.1111/j.1399-302X.1997.tb00375.x

M3 - Article

C2 - 9467404

AN - SCOPUS:0031170995

VL - 12

SP - 174

EP - 177

JO - Molecular Oral Microbiology

JF - Molecular Oral Microbiology

SN - 2041-1006

IS - 3

ER -