Cloning and sequencing of major capsid protein (mcp) gene of a vibriophage, KVP20, possibly related to T-even coliphages

Shigenobu Matsuzaki, Tetsuyoshi Inoue, Masayuki Kuroda, Susumu Kimura, Shuji Tanaka

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Abstract

A large, tailed, prolate-headed vibriophage designated KVP20 was isolated from seawater. KVP20 was morphologically very similar to the previously described vibriophage, KVP40 (Matsuzaki, S., Inoue, T., Tanaka, S, 1998. Virology, 242, 314-318). However, they showed entirely different host specificities and could easily be differentiated from each other by their patterns of DNA restriction fragments. The major capsid protein (mcp) gene of KVP20 encoding the precursor of major capsid protein (pro-Mcp) was cloned and sequenced. The deduced amino-acid (aa) sequence of KVP20 pro-Mcp was compared with the reported aa sequences of KVP40 pro-Mcp, as well as of the equivalent proteins (gp23s) of coliphages T4 and RB49. There was 96.7, 57.5, and 55.2% homology to the corresponding proteins of KVP40, T4, and RB49, respectively. These data strongly suggest that the two vibriophages are closely related to each other and that they are both distantly, but definitely, related to coliphages T4 and RB49.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalGene
Volume222
Issue number1
DOIs
Publication statusPublished - Nov 5 1998

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Keywords

  • Bacteriophage
  • Coliphage T4
  • Evolution
  • Vibrio

ASJC Scopus subject areas

  • Genetics

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