Cloning and sequence analysis of Vibrio parahaemolyticus ompK gene encoding a 26-kDa outer membrane protein, OmpK, that serves as receptor for a broad-host-range vibriophage, KVP40

Tetsuyoshi Inoue, Shigenobu Matsuzaki, Shuji Tanaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The ompK gene of Vibrio parahaemolyticus 1010 (RIMD 2210001) encoding an outer membrane protein (OMP), OmpK, which serves as the receptor for a broad-host-range vibriophage, KVP40, was cloned and sequenced. The gene consisted of 789 nucleotides encoding 263 amino acids. Since the first 20 amino acids most likely constitute the signal peptide, mature OmpK would consist of 243 amino acids with a calculated molecular mass of 27458 Da. Sequence comparisons indicate that OmpK is unique among Vibrio OMPs so far sequenced, but may be distantly related to Tsx of enteric bacteria and is homologous to an Aeromonas hydrophila OMP, protein IV.

Original languageEnglish
Pages (from-to)245-249
Number of pages5
JournalFEMS Microbiology Letters
Volume134
Issue number2-3
DOIs
Publication statusPublished - Dec 15 1995
Externally publishedYes

Fingerprint

Vibrio parahaemolyticus
Host Specificity
Sequence Analysis
Organism Cloning
Membrane Proteins
Amino Acids
Genes
Aeromonas hydrophila
Vibrio
Enterobacteriaceae
Protein Sorting Signals
Nucleotides
Proteins

Keywords

  • OmpK
  • Outer membrane protein
  • Vibrio parahaemolyticus
  • Vibriophage

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Cloning and sequence analysis of Vibrio parahaemolyticus ompK gene encoding a 26-kDa outer membrane protein, OmpK, that serves as receptor for a broad-host-range vibriophage, KVP40. / Inoue, Tetsuyoshi; Matsuzaki, Shigenobu; Tanaka, Shuji.

In: FEMS Microbiology Letters, Vol. 134, No. 2-3, 15.12.1995, p. 245-249.

Research output: Contribution to journalArticle

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