Abstract
The Arabidopsis gene Terminal Flower 1 (TFL1) controls inflorescence meristem identity. A terminal flower (tfl1) mutant, which develops a terminal flower at the apex of the inflorescence, was induced by transformation with T-DNA. Using a plant DNA fragment flanking the integrated T-DNA as a probe, a clone was selected from a wild-type genomic library. Comparative sequence analysis of this clone with an EST clone (129D7T7) suggested the existence of a gene encoding a protein similar to that encoded by the cen gene which controls inflorescence meristem identity in Antirrhinum. Nucleotide sequences of the region homologous to this putative TFL1 gene were compared between five chemically induced tfl1 mutants and their parental wild-type ecotypes. Every mutant was found to have a nucleotide substitution which could be responsible for the tfl1 phenotype. This result confirmed that the cloned gene is TFL1 itself. In our tfl1 mutant, no nucleotide substitution was found in the transcribed region of the gene, and the T-DNA-insertion site was located at 458 bp downstream of the putative polyadenylation signal, suggesting that an element important for expression of the TFL1 gene exists in this area.
| Original language | English |
|---|---|
| Pages (from-to) | 186-194 |
| Number of pages | 9 |
| Journal | MGG Molecular & General Genetics |
| Volume | 254 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1997 |
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Keywords
- Arabidopsis thaliana
- Inflorescence meristem
- T-DNA tagging
- Terminal flower mutant
- TFL1 gene
ASJC Scopus subject areas
- Genetics
Cite this
Cloning and molecular analysis of the Arabidopsis gene Terminal Flower 1. / Ohshima, S.; Murata, M.; Sakamoto, Wataru; Ogura, Y.; Motoyoshi, F.
In: MGG Molecular & General Genetics, Vol. 254, No. 2, 1997, p. 186-194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and molecular analysis of the Arabidopsis gene Terminal Flower 1
AU - Ohshima, S.
AU - Murata, M.
AU - Sakamoto, Wataru
AU - Ogura, Y.
AU - Motoyoshi, F.
PY - 1997
Y1 - 1997
N2 - The Arabidopsis gene Terminal Flower 1 (TFL1) controls inflorescence meristem identity. A terminal flower (tfl1) mutant, which develops a terminal flower at the apex of the inflorescence, was induced by transformation with T-DNA. Using a plant DNA fragment flanking the integrated T-DNA as a probe, a clone was selected from a wild-type genomic library. Comparative sequence analysis of this clone with an EST clone (129D7T7) suggested the existence of a gene encoding a protein similar to that encoded by the cen gene which controls inflorescence meristem identity in Antirrhinum. Nucleotide sequences of the region homologous to this putative TFL1 gene were compared between five chemically induced tfl1 mutants and their parental wild-type ecotypes. Every mutant was found to have a nucleotide substitution which could be responsible for the tfl1 phenotype. This result confirmed that the cloned gene is TFL1 itself. In our tfl1 mutant, no nucleotide substitution was found in the transcribed region of the gene, and the T-DNA-insertion site was located at 458 bp downstream of the putative polyadenylation signal, suggesting that an element important for expression of the TFL1 gene exists in this area.
AB - The Arabidopsis gene Terminal Flower 1 (TFL1) controls inflorescence meristem identity. A terminal flower (tfl1) mutant, which develops a terminal flower at the apex of the inflorescence, was induced by transformation with T-DNA. Using a plant DNA fragment flanking the integrated T-DNA as a probe, a clone was selected from a wild-type genomic library. Comparative sequence analysis of this clone with an EST clone (129D7T7) suggested the existence of a gene encoding a protein similar to that encoded by the cen gene which controls inflorescence meristem identity in Antirrhinum. Nucleotide sequences of the region homologous to this putative TFL1 gene were compared between five chemically induced tfl1 mutants and their parental wild-type ecotypes. Every mutant was found to have a nucleotide substitution which could be responsible for the tfl1 phenotype. This result confirmed that the cloned gene is TFL1 itself. In our tfl1 mutant, no nucleotide substitution was found in the transcribed region of the gene, and the T-DNA-insertion site was located at 458 bp downstream of the putative polyadenylation signal, suggesting that an element important for expression of the TFL1 gene exists in this area.
KW - Arabidopsis thaliana
KW - Inflorescence meristem
KW - T-DNA tagging
KW - Terminal flower mutant
KW - TFL1 gene
UR - http://www.scopus.com/inward/record.url?scp=0030889655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030889655&partnerID=8YFLogxK
U2 - 10.1007/s004380050407
DO - 10.1007/s004380050407
M3 - Article
C2 - 9108281
AN - SCOPUS:0030889655
VL - 254
SP - 186
EP - 194
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 2
ER -