Cloning and Mapping of the Mouse Gpx2 gene Encoding Gastrointestinal Glutathione Peroxidase

Takehito Tsuji, Yuka Watanabe, Hideki Katoh, Katsunori Sato, Tetsuo Kunieda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Gastrointestinal glutathione peroxidase (GSHPx-GI) is an enzyme expressed in intestinal epithelial cells and may reduce hydroperoxides generated from the ingested diet. We isolated a genomic clone containing the mouse Gpx2 gene encoding 190 amino acids of GSHPx-GI. This gene is composed of two exons and an intron. The nucleotide sequence of the coding region was 89.9% identical with that of the human GPX2 gene. A TGA opal codon predicted to encode a selenocysteine was identified at codon 40. A genomic clone containing a pseudogene for the Gpx2 gene was also isolated. The nucleotide sequence of the pseudogene was 98.3% identical with that of the mouse Gpx2 gene and showed characteristics of a processed pseudogene. Linkage analysis using backcross mouse progeny indicated the mouse Gpx2 gene and its pseudogene to be located on mouse chromosomes 12 and 7, respectively.

Original languageEnglish
Pages (from-to)651-654
Number of pages4
JournalJournal of Veterinary Medical Science
Volume60
Issue number5
Publication statusPublished - May 1998

Fingerprint

Glutathione Peroxidase
glutathione peroxidase
Organism Cloning
molecular cloning
Pseudogenes
pseudogenes
mice
Genes
genes
Terminator Codon
Clone Cells
Selenocysteine
clones
selenocysteine
genomics
nucleotide sequences
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 7
stop codon
hydroperoxides

Keywords

  • Chromosome mapping
  • GSHPx-GI
  • Pseudogene

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Cloning and Mapping of the Mouse Gpx2 gene Encoding Gastrointestinal Glutathione Peroxidase. / Tsuji, Takehito; Watanabe, Yuka; Katoh, Hideki; Sato, Katsunori; Kunieda, Tetsuo.

In: Journal of Veterinary Medical Science, Vol. 60, No. 5, 05.1998, p. 651-654.

Research output: Contribution to journalArticle

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AB - Gastrointestinal glutathione peroxidase (GSHPx-GI) is an enzyme expressed in intestinal epithelial cells and may reduce hydroperoxides generated from the ingested diet. We isolated a genomic clone containing the mouse Gpx2 gene encoding 190 amino acids of GSHPx-GI. This gene is composed of two exons and an intron. The nucleotide sequence of the coding region was 89.9% identical with that of the human GPX2 gene. A TGA opal codon predicted to encode a selenocysteine was identified at codon 40. A genomic clone containing a pseudogene for the Gpx2 gene was also isolated. The nucleotide sequence of the pseudogene was 98.3% identical with that of the mouse Gpx2 gene and showed characteristics of a processed pseudogene. Linkage analysis using backcross mouse progeny indicated the mouse Gpx2 gene and its pseudogene to be located on mouse chromosomes 12 and 7, respectively.

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