Cloning and expression of an ether-bond-cleaving enzyme involved in the metabolism of polyethylene glycol

Manabu Yamashita, Akio Tani, Fusako Kawai

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    A gene encoding an ether-bond-cleaving enzyme, diglycolic acid dehydrogenase (DGADH) from polyethylene glycol-utilizing Pseudonocardia sp. strain K1, was cloned and expressed in Escherichia coli. The deduced amino acid sequence had a high homology with superoxide dismutases (SODs) from various bacteria. The recombinant protein showed the same activities as those of DGADH from Pseudonocardia sp. strain K1, namely, SOD activity and ether-bond-cleaving activity.

    Original languageEnglish
    Pages (from-to)313-315
    Number of pages3
    JournalJournal of Bioscience and Bioengineering
    Volume98
    Issue number4
    DOIs
    Publication statusPublished - Oct 2004

    Keywords

    • Ether-bond-cleaving enzyme
    • Metabolism of polyethylene glycol
    • Pseudonocardia sp. strain K1
    • Superoxide dismutase

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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