Cloning and expression of an ether-bond-cleaving enzyme involved in the metabolism of polyethylene glycol

Manabu Yamashita, Akio Tani, Fusako Kawai

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A gene encoding an ether-bond-cleaving enzyme, diglycolic acid dehydrogenase (DGADH) from polyethylene glycol-utilizing Pseudonocardia sp. strain K1, was cloned and expressed in Escherichia coli. The deduced amino acid sequence had a high homology with superoxide dismutases (SODs) from various bacteria. The recombinant protein showed the same activities as those of DGADH from Pseudonocardia sp. strain K1, namely, SOD activity and ether-bond-cleaving activity.

Original languageEnglish
Pages (from-to)313-315
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume98
Issue number4
DOIs
Publication statusPublished - Oct 2004

Keywords

  • Ether-bond-cleaving enzyme
  • Metabolism of polyethylene glycol
  • Pseudonocardia sp. strain K1
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Access to Document

Other files and links

Fingerprint Dive into the research topics of 'Cloning and expression of an ether-bond-cleaving enzyme involved in the metabolism of polyethylene glycol'. Together they form a unique fingerprint.

Cite this