A gene encoding an ether-bond-cleaving enzyme, diglycolic acid dehydrogenase (DGADH) from polyethylene glycol-utilizing Pseudonocardia sp. strain K1, was cloned and expressed in Escherichia coli. The deduced amino acid sequence had a high homology with superoxide dismutases (SODs) from various bacteria. The recombinant protein showed the same activities as those of DGADH from Pseudonocardia sp. strain K1, namely, SOD activity and ether-bond-cleaving activity.
- Ether-bond-cleaving enzyme
- Metabolism of polyethylene glycol
- Pseudonocardia sp. strain K1
- Superoxide dismutase
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology