Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Toru Katase, Yukiko Hoshi, Shotaro Yamaguchi, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

Original languageEnglish
Pages (from-to)1194-1197
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number6
DOIs
Publication statusPublished - Jun 29 2011

Keywords

  • Bacillus circulans
  • Galactooligosaccharide
  • β-galactosidase gene

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Cloning and expression of a β-Galactosidase Gene of Bacillus circulans'. Together they form a unique fingerprint.

Cite this