Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan Song; Hiroyuki Imanaka; Koreyoshi Imamura; Masashi Minoda; Toru Katase; Yukiko Hoshi; Shotaro Yamaguchi; Kazuhiro Nakanishi

doi:10.1271/bbb.110014

Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Toru Katase, Yukiko Hoshi, Shotaro Yamaguchi, Kazuhiro Nakanishi

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

Original language	English
Pages (from-to)	1194-1197
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	75
Issue number	6
DOIs	https://doi.org/10.1271/bbb.110014
Publication status	Published - 2011

Keywords

Bacillus circulans
Galactooligosaccharide
β-galactosidase gene

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.110014

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abstract = "A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta{\textregistered}. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.",

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T1 - Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

AU - Song, Jingyuan

AU - Imanaka, Hiroyuki

AU - Imamura, Koreyoshi

AU - Minoda, Masashi

AU - Katase, Toru

AU - Hoshi, Yukiko

AU - Yamaguchi, Shotaro

AU - Nakanishi, Kazuhiro

PY - 2011

Y1 - 2011

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AB - A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

KW - Bacillus circulans

KW - Galactooligosaccharide

KW - β-galactosidase gene

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JF - Bioscience, Biotechnology and Biochemistry

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ER -

Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Abstract

Keywords

ASJC Scopus subject areas

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