Cloning and expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan Song, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Toru Katase, Yukiko Hoshi, Shotaro Yamaguchi, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

Original languageEnglish
Pages (from-to)1194-1197
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Galactosidases
Cloning
Bacilli
Bacillus
Organism Cloning
Genes
Escherichia coli Proteins
Enzymes
Escherichia coli
Peptides
Proteins

Keywords

  • β-galactosidase gene
  • Bacillus circulans
  • Galactooligosaccharide

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Cloning and expression of a β-Galactosidase Gene of Bacillus circulans. / Song, Jingyuan; Imanaka, Hiroyuki; Imamura, Koreyoshi; Minoda, Masashi; Katase, Toru; Hoshi, Yukiko; Yamaguchi, Shotaro; Nakanishi, Kazuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 6, 2011, p. 1194-1197.

Research output: Contribution to journalArticle

Song, Jingyuan ; Imanaka, Hiroyuki ; Imamura, Koreyoshi ; Minoda, Masashi ; Katase, Toru ; Hoshi, Yukiko ; Yamaguchi, Shotaro ; Nakanishi, Kazuhiro. / Cloning and expression of a β-Galactosidase Gene of Bacillus circulans. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 6. pp. 1194-1197.
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