Cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis): Primary structural features and sequence diversity of the S-RNases in Rosaceae

K. Ushijima, H. Sassa, R. Tao, H. Yamane, A. M. Dandekar, T. M. Gradziel, H. Hirano

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

cDNAs encoding three S-RNases of almond (Prunus dulcis), which belongs to the family Rosaceae, were cloned and sequenced. The comparison of amino acid sequences between the S-RNases of almond and those of other rosaceous species showed that the amino acid sequences of the rosaceous S-RNases are highly divergent, and intra-subfamilial similarities are higher than inter-subfamilial similarities. Twelve amino acid sequences of the rosaceous S-RNases were aligned to characterize their primary structural features. In spite of their high level of diversification, the rosaceous S-RNases were found to have five conserved regions, C1, C2, C3, C5, and RC4 which is Rosaceae-specific conserved region. Many variable sites fall into one region, named RHV. RHV is located at a similar position to that of the hypervariable region a (HVa) of the solanaceous S-RNases, and is assumed to be involved in recognizing S-specificity of pollen. On the other hand, the region corresponding to another solanaceous hypervariable region (HVb) was not variable in the rosaceous S-RNases. In the phylogenetic tree of the T2/S type RNase, the rosaceous S-RNase fall into two subfamily-specific groups (Amygdaloideae and Maloideae). The results of sequence comparisons and phylogenetic analysis imply that the present S-RNases of Rosaceae have diverged again relatively recently, after the divergence of subfamilies.

Original languageEnglish
Pages (from-to)261-268
Number of pages8
JournalMolecular and General Genetics
Volume260
Issue number2-3
DOIs
Publication statusPublished - Dec 14 1998
Externally publishedYes

Keywords

  • Almond
  • Gametophytic self-incompatibility
  • Phylogenetic tree
  • Rosaceae
  • T2/S type RNase

ASJC Scopus subject areas

  • Genetics

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