TY - JOUR
T1 - Cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis)
T2 - Primary structural features and sequence diversity of the S-RNases in Rosaceae
AU - Ushijima, K.
AU - Sassa, H.
AU - Tao, R.
AU - Yamane, H.
AU - Dandekar, A. M.
AU - Gradziel, T. M.
AU - Hirano, H.
N1 - Funding Information:
Acknowledgments We thank Professor Akira Sugiura of Kyoto University for helpful discussion and advice. This work was supported by Kanagawa Academy of Science and Technology Research Grants (H.S) and Grant-in-Aid no. 09460018 for Scientific Research (B) from the Ministry of Education, Science, Sports and Culture of Japan (R.T.).
PY - 1998
Y1 - 1998
N2 - cDNAs encoding three S-RNases of almond (Prunus dulcis), which belongs to the family Rosaceae, were cloned and sequenced. The comparison of amino acid sequences between the S-RNases of almond and those of other rosaceous species showed that the amino acid sequences of the rosaceous S-RNases are highly divergent, and intra-subfamilial similarities are higher than inter-subfamilial similarities. Twelve amino acid sequences of the rosaceous S-RNases were aligned to characterize their primary structural features. In spite of their high level of diversification, the rosaceous S-RNases were found to have five conserved regions, C1, C2, C3, C5, and RC4 which is Rosaceae-specific conserved region. Many variable sites fall into one region, named RHV. RHV is located at a similar position to that of the hypervariable region a (HVa) of the solanaceous S-RNases, and is assumed to be involved in recognizing S-specificity of pollen. On the other hand, the region corresponding to another solanaceous hypervariable region (HVb) was not variable in the rosaceous S-RNases. In the phylogenetic tree of the T2/S type RNase, the rosaceous S-RNase fall into two subfamily-specific groups (Amygdaloideae and Maloideae). The results of sequence comparisons and phylogenetic analysis imply that the present S-RNases of Rosaceae have diverged again relatively recently, after the divergence of subfamilies.
AB - cDNAs encoding three S-RNases of almond (Prunus dulcis), which belongs to the family Rosaceae, were cloned and sequenced. The comparison of amino acid sequences between the S-RNases of almond and those of other rosaceous species showed that the amino acid sequences of the rosaceous S-RNases are highly divergent, and intra-subfamilial similarities are higher than inter-subfamilial similarities. Twelve amino acid sequences of the rosaceous S-RNases were aligned to characterize their primary structural features. In spite of their high level of diversification, the rosaceous S-RNases were found to have five conserved regions, C1, C2, C3, C5, and RC4 which is Rosaceae-specific conserved region. Many variable sites fall into one region, named RHV. RHV is located at a similar position to that of the hypervariable region a (HVa) of the solanaceous S-RNases, and is assumed to be involved in recognizing S-specificity of pollen. On the other hand, the region corresponding to another solanaceous hypervariable region (HVb) was not variable in the rosaceous S-RNases. In the phylogenetic tree of the T2/S type RNase, the rosaceous S-RNase fall into two subfamily-specific groups (Amygdaloideae and Maloideae). The results of sequence comparisons and phylogenetic analysis imply that the present S-RNases of Rosaceae have diverged again relatively recently, after the divergence of subfamilies.
KW - Almond
KW - Gametophytic self-incompatibility
KW - Phylogenetic tree
KW - Rosaceae
KW - T2/S type RNase
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U2 - 10.1007/s004380050894
DO - 10.1007/s004380050894
M3 - Article
C2 - 9862480
AN - SCOPUS:0031765990
VL - 260
SP - 261
EP - 268
JO - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
JF - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
SN - 1617-4615
IS - 2-3
ER -