Cloning and characterization of AN u-glucosidase gene from spinach

Manabu Sugimoto, S. Furui, Y. Suzuki

Research output: Contribution to journalArticle

Abstract

We previously purified and characterized four molecular forms of u-glucosidase from spinach seeds, which have different substrate specificity {Sugimoto etal.,Biosci. Biotech. Biochem. 59: 673-677, 1995). To clarify whether the multiple molecular forms of the enzyme are derived from genetic variation or post-translational modif ication, a cDNA encoding u-glucosidase was isolated from spinach seeds. The cDNA comprised 2867 bp, and included an open reading frame which encodes a polypeptide of 903 amino acid residues. The calculated molecular mass of 101 kDa was larger than those of native u-glucosidases from spinach seeds. The deduced amino acid sequence included those of tryptic peptides from native enzymes. Southern blot analysis of spinach genomic DNA showed a single strong hybridizing band, indicating that the gene which encodes four u-glucosidases in an open reading frame may be as a single-copy gene in spinach. These results suggest that t he multiplicity of u-glucosidase in spin ach occurs via post-translational modification. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan, and by a grant of the Ohara Foundation in Kurashiki with the purpose of promoting the advancement of agricultural sciences.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997

Fingerprint

Glucosidases
glucosidases
Spinacia oleracea
Cloning
spinach
Organism Cloning
molecular cloning
Genes
Seed
Seeds
Organized Financing
genes
Open Reading Frames
open reading frames
Complementary DNA
seeds
science education
Amino Acids
Peptides
post-translational modification

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Cloning and characterization of AN u-glucosidase gene from spinach. / Sugimoto, Manabu; Furui, S.; Suzuki, Y.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

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