Cloning and characterization of an L1 layer-specific gene in Arabidopsis thaliana

Mitsutomo Abe, Taku Takahashi, Yoshibumi Komeda

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The shoot apical meristem (SAM) of Arabidopsis thaliana constitutes the tunica of L1 and L2 and the corpus represented by L3 cells. Regulatory networks involved in establishing and maintaining this structure of shoot meristems remain largely unknown. In order to identify the genes that function in the SAM, we performed cDNA subtraction experiments between wild-type and terminal flower 1 shoot apices. Here, we describe the cloning of a gene designated PDF1 (PROTODERMAL FACTOR1). In situ hybridization revealed that the expression of PDF1 is exclusively limited to the L1 layer of vegetative, inflorescence and floral meristems and to the protoderm of organ primordia. By contrast, PDF1 shows no detectable level of expression in the epidermis of mature organs. Specific expression of the PDF1 gene in protodermal cells is also observed during embryogenesis. The deduced amino acid sequence of PDF1 shares no significant homology with that of other known proteins but contains a putative signal peptide and novel proline-rich repeat motifs, suggesting a cell-wall protein. Possible roles of the PDF1 gene in the SAM are discussed.

Original languageEnglish
Pages (from-to)571-580
Number of pages10
JournalPlant and Cell Physiology
Volume40
Issue number6
Publication statusPublished - Jun 1999
Externally publishedYes

Fingerprint

Meristem
shoot meristems
Arabidopsis
Organism Cloning
molecular cloning
apical meristems
Arabidopsis thaliana
Genes
genes
epidermis (plant)
Inflorescence
signal peptide
meristems
in situ hybridization
proline
Protein Sorting Signals
embryogenesis
inflorescences
amino acid sequences
Proline

Keywords

  • Arabidopsis thaliana
  • CDNA subtraction
  • L1 Layer
  • Tunica-corpus concept

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Cloning and characterization of an L1 layer-specific gene in Arabidopsis thaliana. / Abe, Mitsutomo; Takahashi, Taku; Komeda, Yoshibumi.

In: Plant and Cell Physiology, Vol. 40, No. 6, 06.1999, p. 571-580.

Research output: Contribution to journalArticle

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