Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients

Sabina Mahmood, Miho Sho, Yuko Yasuhara, Miwa Kawanaka, Gouichi Niiyama, Kazumi Togawa, Toshio Ito, Norihiko Takahashi, Moritoshi Kinoshita, Gotaro Yamada

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

Background/aim: Interleukin-8 (IL-8) is known as a chemotactic and angiogenetic cytokine and is a potential mediator of host response to injury or inflammation. In order to identify the role of IL-8 in the pathogenesis of chronic hepatitis C (CHC), we assessed semiquantitatively the messenger RNA (mRNA) expression of IL-8 and other cytokines in liver biopsy specimens of CHC patients. Method: Liver biopsy specimens were obtained under peritoneoscopy from 35 patients with CHC. The mRNA expression of IL-8 and other cytokines in the liver were determined by real-time PCR and the correlation between the mRNA expression and histological classification of liver were studied. Liver histology was classified by both staging of fibrosis (F0-F4) and grading of activity (A1, mild; A2, moderate and A3, severe). Results: Patients were classified into F1, 8; F2, 9; F3, 9 and F4, 9 and A1, 6; A2, 14 and A3, 15, by staging of fibrosis and grading of activity, respectively. Expression of IL-8 mRNA increased with staging of fibrosis (F1, 0.402 ± 0.65; F2, 0.413 ± 0.246; F3, 1.388 ± 2.166; F4, 1.991 ± 1.879) and grading of activity (A1, 0.560 ± 0.808; A2, 0.780 ± 1.268; A3, 1.548 ± 1.957). The mRNA expressions of IL-2, IL-1α, IL-1β, IL-15, and TNF-α were found to be closely correlated with IL-8 mRNA (R = 0.638; 0.522; 0.487; 0.465 and 0.495, respectively, in all P <0.05). Conclusion: In CHC, intra-hepatic expression of both IL-8 and IL-2 increased with fibrosis and inflammatory activity. Positive correlations were found between IL-8 and other cytokines and between cytokines themselves. These findings suggest that these interacting cytokines play an active role in the pathogenesis of CHC, and maybe involved in the upregulation or induction of one and other.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalHepatology Research
Volume24
Issue number4
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

Fingerprint

Chronic Hepatitis C
Interleukin-8
Messenger RNA
Cytokines
Liver
Fibrosis
varespladib methyl
Interleukin-1
Interleukin-2
Biopsy
Interleukin-15
Chemokines
Laparoscopy
Real-Time Polymerase Chain Reaction
Histology
Up-Regulation
Inflammation
Wounds and Injuries

Keywords

  • Chronic hepatitis C
  • Cytokine
  • IL-8
  • Inflammation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Mahmood, S., Sho, M., Yasuhara, Y., Kawanaka, M., Niiyama, G., Togawa, K., ... Yamada, G. (2002). Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients. Hepatology Research, 24(4), 413-419. https://doi.org/10.1016/S1386-6346(02)00136-5

Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients. / Mahmood, Sabina; Sho, Miho; Yasuhara, Yuko; Kawanaka, Miwa; Niiyama, Gouichi; Togawa, Kazumi; Ito, Toshio; Takahashi, Norihiko; Kinoshita, Moritoshi; Yamada, Gotaro.

In: Hepatology Research, Vol. 24, No. 4, 01.12.2002, p. 413-419.

Research output: Contribution to journalReview article

Mahmood, S, Sho, M, Yasuhara, Y, Kawanaka, M, Niiyama, G, Togawa, K, Ito, T, Takahashi, N, Kinoshita, M & Yamada, G 2002, 'Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients', Hepatology Research, vol. 24, no. 4, pp. 413-419. https://doi.org/10.1016/S1386-6346(02)00136-5
Mahmood, Sabina ; Sho, Miho ; Yasuhara, Yuko ; Kawanaka, Miwa ; Niiyama, Gouichi ; Togawa, Kazumi ; Ito, Toshio ; Takahashi, Norihiko ; Kinoshita, Moritoshi ; Yamada, Gotaro. / Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients. In: Hepatology Research. 2002 ; Vol. 24, No. 4. pp. 413-419.
@article{95300aaddfa440f4ada761a306af8875,
title = "Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients",
abstract = "Background/aim: Interleukin-8 (IL-8) is known as a chemotactic and angiogenetic cytokine and is a potential mediator of host response to injury or inflammation. In order to identify the role of IL-8 in the pathogenesis of chronic hepatitis C (CHC), we assessed semiquantitatively the messenger RNA (mRNA) expression of IL-8 and other cytokines in liver biopsy specimens of CHC patients. Method: Liver biopsy specimens were obtained under peritoneoscopy from 35 patients with CHC. The mRNA expression of IL-8 and other cytokines in the liver were determined by real-time PCR and the correlation between the mRNA expression and histological classification of liver were studied. Liver histology was classified by both staging of fibrosis (F0-F4) and grading of activity (A1, mild; A2, moderate and A3, severe). Results: Patients were classified into F1, 8; F2, 9; F3, 9 and F4, 9 and A1, 6; A2, 14 and A3, 15, by staging of fibrosis and grading of activity, respectively. Expression of IL-8 mRNA increased with staging of fibrosis (F1, 0.402 ± 0.65; F2, 0.413 ± 0.246; F3, 1.388 ± 2.166; F4, 1.991 ± 1.879) and grading of activity (A1, 0.560 ± 0.808; A2, 0.780 ± 1.268; A3, 1.548 ± 1.957). The mRNA expressions of IL-2, IL-1α, IL-1β, IL-15, and TNF-α were found to be closely correlated with IL-8 mRNA (R = 0.638; 0.522; 0.487; 0.465 and 0.495, respectively, in all P <0.05). Conclusion: In CHC, intra-hepatic expression of both IL-8 and IL-2 increased with fibrosis and inflammatory activity. Positive correlations were found between IL-8 and other cytokines and between cytokines themselves. These findings suggest that these interacting cytokines play an active role in the pathogenesis of CHC, and maybe involved in the upregulation or induction of one and other.",
keywords = "Chronic hepatitis C, Cytokine, IL-8, Inflammation",
author = "Sabina Mahmood and Miho Sho and Yuko Yasuhara and Miwa Kawanaka and Gouichi Niiyama and Kazumi Togawa and Toshio Ito and Norihiko Takahashi and Moritoshi Kinoshita and Gotaro Yamada",
year = "2002",
month = "12",
day = "1",
doi = "10.1016/S1386-6346(02)00136-5",
language = "English",
volume = "24",
pages = "413--419",
journal = "Hepatology Research",
issn = "1386-6346",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Clinical significance of intrahepatic interleukin-8 in chronic hepatitis C patients

AU - Mahmood, Sabina

AU - Sho, Miho

AU - Yasuhara, Yuko

AU - Kawanaka, Miwa

AU - Niiyama, Gouichi

AU - Togawa, Kazumi

AU - Ito, Toshio

AU - Takahashi, Norihiko

AU - Kinoshita, Moritoshi

AU - Yamada, Gotaro

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Background/aim: Interleukin-8 (IL-8) is known as a chemotactic and angiogenetic cytokine and is a potential mediator of host response to injury or inflammation. In order to identify the role of IL-8 in the pathogenesis of chronic hepatitis C (CHC), we assessed semiquantitatively the messenger RNA (mRNA) expression of IL-8 and other cytokines in liver biopsy specimens of CHC patients. Method: Liver biopsy specimens were obtained under peritoneoscopy from 35 patients with CHC. The mRNA expression of IL-8 and other cytokines in the liver were determined by real-time PCR and the correlation between the mRNA expression and histological classification of liver were studied. Liver histology was classified by both staging of fibrosis (F0-F4) and grading of activity (A1, mild; A2, moderate and A3, severe). Results: Patients were classified into F1, 8; F2, 9; F3, 9 and F4, 9 and A1, 6; A2, 14 and A3, 15, by staging of fibrosis and grading of activity, respectively. Expression of IL-8 mRNA increased with staging of fibrosis (F1, 0.402 ± 0.65; F2, 0.413 ± 0.246; F3, 1.388 ± 2.166; F4, 1.991 ± 1.879) and grading of activity (A1, 0.560 ± 0.808; A2, 0.780 ± 1.268; A3, 1.548 ± 1.957). The mRNA expressions of IL-2, IL-1α, IL-1β, IL-15, and TNF-α were found to be closely correlated with IL-8 mRNA (R = 0.638; 0.522; 0.487; 0.465 and 0.495, respectively, in all P <0.05). Conclusion: In CHC, intra-hepatic expression of both IL-8 and IL-2 increased with fibrosis and inflammatory activity. Positive correlations were found between IL-8 and other cytokines and between cytokines themselves. These findings suggest that these interacting cytokines play an active role in the pathogenesis of CHC, and maybe involved in the upregulation or induction of one and other.

AB - Background/aim: Interleukin-8 (IL-8) is known as a chemotactic and angiogenetic cytokine and is a potential mediator of host response to injury or inflammation. In order to identify the role of IL-8 in the pathogenesis of chronic hepatitis C (CHC), we assessed semiquantitatively the messenger RNA (mRNA) expression of IL-8 and other cytokines in liver biopsy specimens of CHC patients. Method: Liver biopsy specimens were obtained under peritoneoscopy from 35 patients with CHC. The mRNA expression of IL-8 and other cytokines in the liver were determined by real-time PCR and the correlation between the mRNA expression and histological classification of liver were studied. Liver histology was classified by both staging of fibrosis (F0-F4) and grading of activity (A1, mild; A2, moderate and A3, severe). Results: Patients were classified into F1, 8; F2, 9; F3, 9 and F4, 9 and A1, 6; A2, 14 and A3, 15, by staging of fibrosis and grading of activity, respectively. Expression of IL-8 mRNA increased with staging of fibrosis (F1, 0.402 ± 0.65; F2, 0.413 ± 0.246; F3, 1.388 ± 2.166; F4, 1.991 ± 1.879) and grading of activity (A1, 0.560 ± 0.808; A2, 0.780 ± 1.268; A3, 1.548 ± 1.957). The mRNA expressions of IL-2, IL-1α, IL-1β, IL-15, and TNF-α were found to be closely correlated with IL-8 mRNA (R = 0.638; 0.522; 0.487; 0.465 and 0.495, respectively, in all P <0.05). Conclusion: In CHC, intra-hepatic expression of both IL-8 and IL-2 increased with fibrosis and inflammatory activity. Positive correlations were found between IL-8 and other cytokines and between cytokines themselves. These findings suggest that these interacting cytokines play an active role in the pathogenesis of CHC, and maybe involved in the upregulation or induction of one and other.

KW - Chronic hepatitis C

KW - Cytokine

KW - IL-8

KW - Inflammation

UR - http://www.scopus.com/inward/record.url?scp=0036911194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036911194&partnerID=8YFLogxK

U2 - 10.1016/S1386-6346(02)00136-5

DO - 10.1016/S1386-6346(02)00136-5

M3 - Review article

VL - 24

SP - 413

EP - 419

JO - Hepatology Research

JF - Hepatology Research

SN - 1386-6346

IS - 4

ER -