Clinical outcomes of MII oocytes with refractile bodies in patients undergoing ICSI and single frozen embryo transfer

Hiromi Takahashi, Junko Otsuki, Michio Yamamoto, Hiroe Saito, Rei Hirata, Toshihiro Habara, Nobuyoshi Hayashi

Research output: Contribution to journalArticle

Abstract

Purpose: This study aimed to analyze whether the presence of refractile bodies (RFs) negatively affects fertilization, embryo development, and/or implantation rates following intracytoplasmic sperm injection (ICSI). Methods: This retrospective embryo cohort study involved a total of 272 patients undergoing ICSI treatment of blastocyst cryopreservation. Results: In the study, no significant differences were found regarding 2PN formation rates between RF(+) (76.5%) and RF(−) oocytes (77.2%). However, the blastocyst formation rate on Day 5 in RF(+) oocytes was 45.8%, which was significantly lower than that of 52.2% in RF(−) oocytes (aOR 0.74, 95% CI 0.59-0.93, P =.011). Implantation rates were also significantly lower in RF(+) oocytes (24.2%) as compared to RF(−) oocytes (42.2%) (aOR 0.46, 95% CI 0.26-0.78, P =.005). Furthermore, the implantation rate of RF(+) oocytes (28.6%), when high-quality blastocysts were transferred, was significantly lower than that of RF(−) oocytes (46.1%) (aOR 0.50, 95% CI 0.25-0.96, P =.043). Conclusion: Our results suggest that oocytes with the presence of RFs have a lower potential for blastocyst development. Even when they develop into high-quality blastocysts, the chances of implantation are reduced.

Original languageEnglish
JournalReproductive Medicine and Biology
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Single Embryo Transfer
Intracytoplasmic Sperm Injections
Oocytes
Blastocyst
Cryopreservation
Fertilization
Embryonic Development
Cohort Studies
Embryonic Structures

Keywords

  • blastocyst
  • cytoplasmic morphology
  • embryo implantation
  • ICSI
  • refractile body

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Clinical outcomes of MII oocytes with refractile bodies in patients undergoing ICSI and single frozen embryo transfer. / Takahashi, Hiromi; Otsuki, Junko; Yamamoto, Michio; Saito, Hiroe; Hirata, Rei; Habara, Toshihiro; Hayashi, Nobuyoshi.

In: Reproductive Medicine and Biology, 01.01.2019.

Research output: Contribution to journalArticle

Takahashi, Hiromi ; Otsuki, Junko ; Yamamoto, Michio ; Saito, Hiroe ; Hirata, Rei ; Habara, Toshihiro ; Hayashi, Nobuyoshi. / Clinical outcomes of MII oocytes with refractile bodies in patients undergoing ICSI and single frozen embryo transfer. In: Reproductive Medicine and Biology. 2019.
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abstract = "Purpose: This study aimed to analyze whether the presence of refractile bodies (RFs) negatively affects fertilization, embryo development, and/or implantation rates following intracytoplasmic sperm injection (ICSI). Methods: This retrospective embryo cohort study involved a total of 272 patients undergoing ICSI treatment of blastocyst cryopreservation. Results: In the study, no significant differences were found regarding 2PN formation rates between RF(+) (76.5{\%}) and RF(−) oocytes (77.2{\%}). However, the blastocyst formation rate on Day 5 in RF(+) oocytes was 45.8{\%}, which was significantly lower than that of 52.2{\%} in RF(−) oocytes (aOR 0.74, 95{\%} CI 0.59-0.93, P =.011). Implantation rates were also significantly lower in RF(+) oocytes (24.2{\%}) as compared to RF(−) oocytes (42.2{\%}) (aOR 0.46, 95{\%} CI 0.26-0.78, P =.005). Furthermore, the implantation rate of RF(+) oocytes (28.6{\%}), when high-quality blastocysts were transferred, was significantly lower than that of RF(−) oocytes (46.1{\%}) (aOR 0.50, 95{\%} CI 0.25-0.96, P =.043). Conclusion: Our results suggest that oocytes with the presence of RFs have a lower potential for blastocyst development. Even when they develop into high-quality blastocysts, the chances of implantation are reduced.",
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AU - Otsuki, Junko

AU - Yamamoto, Michio

AU - Saito, Hiroe

AU - Hirata, Rei

AU - Habara, Toshihiro

AU - Hayashi, Nobuyoshi

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N2 - Purpose: This study aimed to analyze whether the presence of refractile bodies (RFs) negatively affects fertilization, embryo development, and/or implantation rates following intracytoplasmic sperm injection (ICSI). Methods: This retrospective embryo cohort study involved a total of 272 patients undergoing ICSI treatment of blastocyst cryopreservation. Results: In the study, no significant differences were found regarding 2PN formation rates between RF(+) (76.5%) and RF(−) oocytes (77.2%). However, the blastocyst formation rate on Day 5 in RF(+) oocytes was 45.8%, which was significantly lower than that of 52.2% in RF(−) oocytes (aOR 0.74, 95% CI 0.59-0.93, P =.011). Implantation rates were also significantly lower in RF(+) oocytes (24.2%) as compared to RF(−) oocytes (42.2%) (aOR 0.46, 95% CI 0.26-0.78, P =.005). Furthermore, the implantation rate of RF(+) oocytes (28.6%), when high-quality blastocysts were transferred, was significantly lower than that of RF(−) oocytes (46.1%) (aOR 0.50, 95% CI 0.25-0.96, P =.043). Conclusion: Our results suggest that oocytes with the presence of RFs have a lower potential for blastocyst development. Even when they develop into high-quality blastocysts, the chances of implantation are reduced.

AB - Purpose: This study aimed to analyze whether the presence of refractile bodies (RFs) negatively affects fertilization, embryo development, and/or implantation rates following intracytoplasmic sperm injection (ICSI). Methods: This retrospective embryo cohort study involved a total of 272 patients undergoing ICSI treatment of blastocyst cryopreservation. Results: In the study, no significant differences were found regarding 2PN formation rates between RF(+) (76.5%) and RF(−) oocytes (77.2%). However, the blastocyst formation rate on Day 5 in RF(+) oocytes was 45.8%, which was significantly lower than that of 52.2% in RF(−) oocytes (aOR 0.74, 95% CI 0.59-0.93, P =.011). Implantation rates were also significantly lower in RF(+) oocytes (24.2%) as compared to RF(−) oocytes (42.2%) (aOR 0.46, 95% CI 0.26-0.78, P =.005). Furthermore, the implantation rate of RF(+) oocytes (28.6%), when high-quality blastocysts were transferred, was significantly lower than that of RF(−) oocytes (46.1%) (aOR 0.50, 95% CI 0.25-0.96, P =.043). Conclusion: Our results suggest that oocytes with the presence of RFs have a lower potential for blastocyst development. Even when they develop into high-quality blastocysts, the chances of implantation are reduced.

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KW - cytoplasmic morphology

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