TY - JOUR
T1 - Clinical outcomes of MII oocytes with refractile bodies in patients undergoing ICSI and single frozen embryo transfer
AU - Takahashi, Hiromi
AU - Otsuki, Junko
AU - Yamamoto, Michio
AU - Saito, Hiroe
AU - Hirata, Rei
AU - Habara, Toshihiro
AU - Hayashi, Nobuyoshi
N1 - Publisher Copyright:
© 2019 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Purpose: This study aimed to analyze whether the presence of refractile bodies (RFs) negatively affects fertilization, embryo development, and/or implantation rates following intracytoplasmic sperm injection (ICSI). Methods: This retrospective embryo cohort study involved a total of 272 patients undergoing ICSI treatment of blastocyst cryopreservation. Results: In the study, no significant differences were found regarding 2PN formation rates between RF(+) (76.5%) and RF(−) oocytes (77.2%). However, the blastocyst formation rate on Day 5 in RF(+) oocytes was 45.8%, which was significantly lower than that of 52.2% in RF(−) oocytes (aOR 0.74, 95% CI 0.59-0.93, P =.011). Implantation rates were also significantly lower in RF(+) oocytes (24.2%) as compared to RF(−) oocytes (42.2%) (aOR 0.46, 95% CI 0.26-0.78, P =.005). Furthermore, the implantation rate of RF(+) oocytes (28.6%), when high-quality blastocysts were transferred, was significantly lower than that of RF(−) oocytes (46.1%) (aOR 0.50, 95% CI 0.25-0.96, P =.043). Conclusion: Our results suggest that oocytes with the presence of RFs have a lower potential for blastocyst development. Even when they develop into high-quality blastocysts, the chances of implantation are reduced.
AB - Purpose: This study aimed to analyze whether the presence of refractile bodies (RFs) negatively affects fertilization, embryo development, and/or implantation rates following intracytoplasmic sperm injection (ICSI). Methods: This retrospective embryo cohort study involved a total of 272 patients undergoing ICSI treatment of blastocyst cryopreservation. Results: In the study, no significant differences were found regarding 2PN formation rates between RF(+) (76.5%) and RF(−) oocytes (77.2%). However, the blastocyst formation rate on Day 5 in RF(+) oocytes was 45.8%, which was significantly lower than that of 52.2% in RF(−) oocytes (aOR 0.74, 95% CI 0.59-0.93, P =.011). Implantation rates were also significantly lower in RF(+) oocytes (24.2%) as compared to RF(−) oocytes (42.2%) (aOR 0.46, 95% CI 0.26-0.78, P =.005). Furthermore, the implantation rate of RF(+) oocytes (28.6%), when high-quality blastocysts were transferred, was significantly lower than that of RF(−) oocytes (46.1%) (aOR 0.50, 95% CI 0.25-0.96, P =.043). Conclusion: Our results suggest that oocytes with the presence of RFs have a lower potential for blastocyst development. Even when they develop into high-quality blastocysts, the chances of implantation are reduced.
KW - ICSI
KW - blastocyst
KW - cytoplasmic morphology
KW - embryo implantation
KW - refractile body
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U2 - 10.1002/rmb2.12305
DO - 10.1002/rmb2.12305
M3 - Article
AN - SCOPUS:85075468084
VL - 19
SP - 75
EP - 81
JO - Reproductive Medicine and Biology
JF - Reproductive Medicine and Biology
SN - 1445-5781
IS - 1
ER -