Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation

Noriko Doki, Masahiro Suyama, Satoshi Sasajima, Junko Ota, Aiko Igarashi, Iyo Mimura, Hidetoshi Morita, Yuki Fujioka, Daisuke Sugiyama, Hiroyoshi Nishikawa, Yutaka Shimazu, Wataru Suda, Kozue Takeshita, Koji Atarashi, Masahira Hattori, Eiichi Sato, Kyoko Watakabe-Inamoto, Kosuke Yoshioka, Yuho Najima, Takeshi Kobayashi & 5 others Kazuhiko Kakihana, Naoto Takahashi, Hisashi Sakamaki, Kenya Honda, Kazuteru Ohashi

Research output: Contribution to journalArticle

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Abstract

Post-transplant microbial diversity in the gastrointestinal tract is closely associated with clinical outcomes following allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, little is known about the impact of the fecal microbiota before allo-HSCT. We analyzed fecal samples approximately 2 weeks before conditioning among 107 allo-HSCT recipients between 2013 and 2015. Microbial analysis was performed using 16S rRNA gene sequencing. Operational taxonomic unit-based microbial diversity was estimated by calculating the Shannon index. Patients were classified into three groups based on the diversity index: low (<2), intermediate (2, 3), and high (>3) diversity (18 (16.8%), 48 (44.9%), and 41 (38.3%) patients, respectively). There were no significant differences in the 20-month overall survival, cumulative incidence of relapse, and non-relapse mortality among three groups. The cumulative incidence of grade II to IV acute graft-versus-host disease (aGVHD) was similar among the three groups (low 55.6%; intermediate 35.4%; high 48.8%, p = 0.339, at day 100). Furthermore, we found no differences in the cumulative incidence of grade II to IV acute gastrointestinal GVHD among the three groups (low 38.9%; intermediate 21.3%; high 24.4%, p = 0.778, at day 100). Regarding the composition of microbiota before allo-HSCT, aGVHD patients showed a significantly higher abundance of phylum Firmicutes (p < 0.01) and a lower tendency for Bacteroidetes (p = 0.106) than non-aGVHD patients. Maintenance of Bacteroidetes throughout allo-HSCT may be a strategy to prevent aGVHD.

Original languageEnglish
Pages (from-to)1517-1523
Number of pages7
JournalAnnals of Hematology
Volume96
Issue number9
DOIs
Publication statusPublished - Sep 1 2017

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Hematopoietic Stem Cell Transplantation
Graft vs Host Disease
Transplants
Bacteroidetes
Microbiota
Incidence
rRNA Genes
Gastrointestinal Tract
Maintenance
Recurrence
Survival
Mortality

Keywords

  • Acute gastrointestinal graft-versus-host disease (GVHD)
  • Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
  • Intestinal microbiota
  • Microbial diversity

ASJC Scopus subject areas

  • Hematology

Cite this

Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation. / Doki, Noriko; Suyama, Masahiro; Sasajima, Satoshi; Ota, Junko; Igarashi, Aiko; Mimura, Iyo; Morita, Hidetoshi; Fujioka, Yuki; Sugiyama, Daisuke; Nishikawa, Hiroyoshi; Shimazu, Yutaka; Suda, Wataru; Takeshita, Kozue; Atarashi, Koji; Hattori, Masahira; Sato, Eiichi; Watakabe-Inamoto, Kyoko; Yoshioka, Kosuke; Najima, Yuho; Kobayashi, Takeshi; Kakihana, Kazuhiko; Takahashi, Naoto; Sakamaki, Hisashi; Honda, Kenya; Ohashi, Kazuteru.

In: Annals of Hematology, Vol. 96, No. 9, 01.09.2017, p. 1517-1523.

Research output: Contribution to journalArticle

Doki, N, Suyama, M, Sasajima, S, Ota, J, Igarashi, A, Mimura, I, Morita, H, Fujioka, Y, Sugiyama, D, Nishikawa, H, Shimazu, Y, Suda, W, Takeshita, K, Atarashi, K, Hattori, M, Sato, E, Watakabe-Inamoto, K, Yoshioka, K, Najima, Y, Kobayashi, T, Kakihana, K, Takahashi, N, Sakamaki, H, Honda, K & Ohashi, K 2017, 'Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation', Annals of Hematology, vol. 96, no. 9, pp. 1517-1523. https://doi.org/10.1007/s00277-017-3069-8
Doki, Noriko ; Suyama, Masahiro ; Sasajima, Satoshi ; Ota, Junko ; Igarashi, Aiko ; Mimura, Iyo ; Morita, Hidetoshi ; Fujioka, Yuki ; Sugiyama, Daisuke ; Nishikawa, Hiroyoshi ; Shimazu, Yutaka ; Suda, Wataru ; Takeshita, Kozue ; Atarashi, Koji ; Hattori, Masahira ; Sato, Eiichi ; Watakabe-Inamoto, Kyoko ; Yoshioka, Kosuke ; Najima, Yuho ; Kobayashi, Takeshi ; Kakihana, Kazuhiko ; Takahashi, Naoto ; Sakamaki, Hisashi ; Honda, Kenya ; Ohashi, Kazuteru. / Clinical impact of pre-transplant gut microbial diversity on outcomes of allogeneic hematopoietic stem cell transplantation. In: Annals of Hematology. 2017 ; Vol. 96, No. 9. pp. 1517-1523.
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