Clinical, histopathological, and immunogenetic analysis of ocular adnexal lymphoproliferative disorders: Characterization of MALT lymphoma and reactive lymphoid hyperplasia

Tomohiko Mannami, Tadashi Yoshino, Koichi Oshima, Sumie Takase, Eisaku Kondo, Nobuya Ohara, Hideki Nakagawa, Hiroshi Ohtsuki, Mine Harada, Tadaatsu Akagi

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Malignant lymphomas and reactive lymphoi hyperplasia (RLH) in the ocular adnexa are sometimes difficult to differentiate morphologically and have often been categorized together as a lymphoproliferative disorder. Immunogenotypic characters of these diseases have not yet been well clarified. This study included 76 cases of ocular adnexal lymphoproliferative disorders. These consisted of 52 cases of malignant lymphoma (43 primary and secondary), 22 of RLH, and 2 borderline cases. There were slightly more male than female subjects. Diagnoses were based on morphology and immunophenotypic characteristics. Clonalities were detected by means of polymerase chain reaction (PCR), an immunoglobulin heavy-chain variable region (VH) genes were sequenced in 10 cases of mucosa-associated lymphoid tissue (MALT) lymphoma. MAL lymphoma constituted 86% (37 cases) of the primary lymphomas. MALT lymphomas were more indolent, more rarely disseminated, and had a lower death rate than the other primary lymphomas. Two patients exhibited coexistence of MALT and diffuse large B-cell lymphoma. The average age of patients with RLH was 5.5 years younger than that of those with MALT lymphoma. One of the cases of RLH later progressed to malignant lymphoma. B-cell clonality was detected by PCR in 57%, 55%, and 0% o primary lymphomas, MALT lymphomas and RLHs respectively. Sequencing of VH genes revealed that the VH3 family was the most commonly expressed germline VH family (70%) and that DP-63, DP-54 and DP-47 genes were frequently found in the MALT lymphomas examined. PCR analysis wa useful for differentiation between MALT lymphoma and RLH. Sequence analysis of VH genes showed that an autoimmune mechanism may be involved in the lymphomagenesis of ocular adnexal MALT lymphoma.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalModern Pathology
Volume14
Issue number7
DOIs
Publication statusPublished - 2001

Fingerprint

Pseudolymphoma
Immunogenetics
Marginal Zone B-Cell Lymphoma
Lymphoproliferative Disorders
Lymphoma
Hyperplasia
Polymerase Chain Reaction
Genes
Immunoglobulin Heavy Chains
Lymphoma, Large B-Cell, Diffuse
Lymphoid Tissue
Sequence Analysis
Mucous Membrane
B-Lymphocytes
Mortality

Keywords

  • Differential diagnosis
  • Immunogenetic analysis
  • MALT lymphoma
  • Ocular adnexa
  • PCR
  • Reactive lymphoid hyperplasia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Clinical, histopathological, and immunogenetic analysis of ocular adnexal lymphoproliferative disorders : Characterization of MALT lymphoma and reactive lymphoid hyperplasia. / Mannami, Tomohiko; Yoshino, Tadashi; Oshima, Koichi; Takase, Sumie; Kondo, Eisaku; Ohara, Nobuya; Nakagawa, Hideki; Ohtsuki, Hiroshi; Harada, Mine; Akagi, Tadaatsu.

In: Modern Pathology, Vol. 14, No. 7, 2001, p. 641-649.

Research output: Contribution to journalArticle

Mannami, Tomohiko ; Yoshino, Tadashi ; Oshima, Koichi ; Takase, Sumie ; Kondo, Eisaku ; Ohara, Nobuya ; Nakagawa, Hideki ; Ohtsuki, Hiroshi ; Harada, Mine ; Akagi, Tadaatsu. / Clinical, histopathological, and immunogenetic analysis of ocular adnexal lymphoproliferative disorders : Characterization of MALT lymphoma and reactive lymphoid hyperplasia. In: Modern Pathology. 2001 ; Vol. 14, No. 7. pp. 641-649.
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AU - Takase, Sumie

AU - Kondo, Eisaku

AU - Ohara, Nobuya

AU - Nakagawa, Hideki

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AU - Harada, Mine

AU - Akagi, Tadaatsu

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KW - PCR

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