Abstract
Malignant lymphomas and reactive lymphoi hyperplasia (RLH) in the ocular adnexa are sometimes difficult to differentiate morphologically and have often been categorized together as a lymphoproliferative disorder. Immunogenotypic characters of these diseases have not yet been well clarified. This study included 76 cases of ocular adnexal lymphoproliferative disorders. These consisted of 52 cases of malignant lymphoma (43 primary and secondary), 22 of RLH, and 2 borderline cases. There were slightly more male than female subjects. Diagnoses were based on morphology and immunophenotypic characteristics. Clonalities were detected by means of polymerase chain reaction (PCR), an immunoglobulin heavy-chain variable region (VH) genes were sequenced in 10 cases of mucosa-associated lymphoid tissue (MALT) lymphoma. MAL lymphoma constituted 86% (37 cases) of the primary lymphomas. MALT lymphomas were more indolent, more rarely disseminated, and had a lower death rate than the other primary lymphomas. Two patients exhibited coexistence of MALT and diffuse large B-cell lymphoma. The average age of patients with RLH was 5.5 years younger than that of those with MALT lymphoma. One of the cases of RLH later progressed to malignant lymphoma. B-cell clonality was detected by PCR in 57%, 55%, and 0% o primary lymphomas, MALT lymphomas and RLHs respectively. Sequencing of VH genes revealed that the VH3 family was the most commonly expressed germline VH family (70%) and that DP-63, DP-54 and DP-47 genes were frequently found in the MALT lymphomas examined. PCR analysis wa useful for differentiation between MALT lymphoma and RLH. Sequence analysis of VH genes showed that an autoimmune mechanism may be involved in the lymphomagenesis of ocular adnexal MALT lymphoma.
| Original language | English |
|---|---|
| Pages (from-to) | 641-649 |
| Number of pages | 9 |
| Journal | Modern Pathology |
| Volume | 14 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Differential diagnosis
- Immunogenetic analysis
- MALT lymphoma
- Ocular adnexa
- PCR
- Reactive lymphoid hyperplasia
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Clinical, histopathological, and immunogenetic analysis of ocular adnexal lymphoproliferative disorders : Characterization of MALT lymphoma and reactive lymphoid hyperplasia. / Mannami, Tomohiko; Yoshino, Tadashi; Oshima, Koichi; Takase, Sumie; Kondo, Eisaku; Ohara, Nobuya; Nakagawa, Hideki; Ohtsuki, Hiroshi; Harada, Mine; Akagi, Tadaatsu.
In: Modern Pathology, Vol. 14, No. 7, 2001, p. 641-649.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical, histopathological, and immunogenetic analysis of ocular adnexal lymphoproliferative disorders
T2 - Characterization of MALT lymphoma and reactive lymphoid hyperplasia
AU - Mannami, Tomohiko
AU - Yoshino, Tadashi
AU - Oshima, Koichi
AU - Takase, Sumie
AU - Kondo, Eisaku
AU - Ohara, Nobuya
AU - Nakagawa, Hideki
AU - Ohtsuki, Hiroshi
AU - Harada, Mine
AU - Akagi, Tadaatsu
PY - 2001
Y1 - 2001
N2 - Malignant lymphomas and reactive lymphoi hyperplasia (RLH) in the ocular adnexa are sometimes difficult to differentiate morphologically and have often been categorized together as a lymphoproliferative disorder. Immunogenotypic characters of these diseases have not yet been well clarified. This study included 76 cases of ocular adnexal lymphoproliferative disorders. These consisted of 52 cases of malignant lymphoma (43 primary and secondary), 22 of RLH, and 2 borderline cases. There were slightly more male than female subjects. Diagnoses were based on morphology and immunophenotypic characteristics. Clonalities were detected by means of polymerase chain reaction (PCR), an immunoglobulin heavy-chain variable region (VH) genes were sequenced in 10 cases of mucosa-associated lymphoid tissue (MALT) lymphoma. MAL lymphoma constituted 86% (37 cases) of the primary lymphomas. MALT lymphomas were more indolent, more rarely disseminated, and had a lower death rate than the other primary lymphomas. Two patients exhibited coexistence of MALT and diffuse large B-cell lymphoma. The average age of patients with RLH was 5.5 years younger than that of those with MALT lymphoma. One of the cases of RLH later progressed to malignant lymphoma. B-cell clonality was detected by PCR in 57%, 55%, and 0% o primary lymphomas, MALT lymphomas and RLHs respectively. Sequencing of VH genes revealed that the VH3 family was the most commonly expressed germline VH family (70%) and that DP-63, DP-54 and DP-47 genes were frequently found in the MALT lymphomas examined. PCR analysis wa useful for differentiation between MALT lymphoma and RLH. Sequence analysis of VH genes showed that an autoimmune mechanism may be involved in the lymphomagenesis of ocular adnexal MALT lymphoma.
AB - Malignant lymphomas and reactive lymphoi hyperplasia (RLH) in the ocular adnexa are sometimes difficult to differentiate morphologically and have often been categorized together as a lymphoproliferative disorder. Immunogenotypic characters of these diseases have not yet been well clarified. This study included 76 cases of ocular adnexal lymphoproliferative disorders. These consisted of 52 cases of malignant lymphoma (43 primary and secondary), 22 of RLH, and 2 borderline cases. There were slightly more male than female subjects. Diagnoses were based on morphology and immunophenotypic characteristics. Clonalities were detected by means of polymerase chain reaction (PCR), an immunoglobulin heavy-chain variable region (VH) genes were sequenced in 10 cases of mucosa-associated lymphoid tissue (MALT) lymphoma. MAL lymphoma constituted 86% (37 cases) of the primary lymphomas. MALT lymphomas were more indolent, more rarely disseminated, and had a lower death rate than the other primary lymphomas. Two patients exhibited coexistence of MALT and diffuse large B-cell lymphoma. The average age of patients with RLH was 5.5 years younger than that of those with MALT lymphoma. One of the cases of RLH later progressed to malignant lymphoma. B-cell clonality was detected by PCR in 57%, 55%, and 0% o primary lymphomas, MALT lymphomas and RLHs respectively. Sequencing of VH genes revealed that the VH3 family was the most commonly expressed germline VH family (70%) and that DP-63, DP-54 and DP-47 genes were frequently found in the MALT lymphomas examined. PCR analysis wa useful for differentiation between MALT lymphoma and RLH. Sequence analysis of VH genes showed that an autoimmune mechanism may be involved in the lymphomagenesis of ocular adnexal MALT lymphoma.
KW - Differential diagnosis
KW - Immunogenetic analysis
KW - MALT lymphoma
KW - Ocular adnexa
KW - PCR
KW - Reactive lymphoid hyperplasia
UR - http://www.scopus.com/inward/record.url?scp=17844392607&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17844392607&partnerID=8YFLogxK
U2 - 10.1038/modpathol.3880366
DO - 10.1038/modpathol.3880366
M3 - Article
C2 - 11454995
AN - SCOPUS:17844392607
VL - 14
SP - 641
EP - 649
JO - Modern Pathology
JF - Modern Pathology
SN - 0893-3952
IS - 7
ER -