Clinical evaluation of the efficacy of removing microorganisms to disinfect patient-derived dental impressions

Hiroshi Egusa, Takao Watamoto, Takuya Matsumoto, Keiko Abe, Munemasa Kobayashi, Yoshihiro Akashi, Hirofumi Yatani

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Disinfection of dental impressions is an indispensable procedure for the control of cross-contamination; however, there is limited information on the efficacy of disinfection under clinical conditions. The objective of this study was to clinically evaluate the disinfection efficacy of commercially available agents in removing oral pathogens from patient-derived impressions. Materials and Methods: Impressions from 54 patients were divided into groups and either left undisinfected or underwent 1 of 5 disinfection treatments: (1) 2% glutaraldehyde (GA), (2) 1% sodium hypochlorite (SH), (3) 0.25% benzalkonium chloride (BC), (4) 1 ppm ozonated water (OW), or (5) the Hygojet/MD520 system (HJ). An impression culture technique using a brain heart infusion agar medium was used to visualize the microbial contamination on the surface of the impression cultures. The persistent presence of oral pathogens on the impression cultures was examined using selective isolation agar plates. Results: The isolation frequencies of streptococci, staphylococci, Candida, methicillin-resistant Staphylococcus aureus, and Pseudomonas aeruginosa species from undisinfected impressions were 100%, 55.6%, 25.9%, 25.9% and 5.6%, respectively. Disinfection with HJ and BC removed the microorganisms with the greatest efficacy, followed by GA, SH, and OW. Potential bacterial contamination could be detected even after disinfection had been performed. Combined use of BC plus GA or SH removed oral pathogens almost completely from dental impressions. Conclusions: This investigation showed that potential contaminants are still present, even after general disinfection procedures. Therefore, either HJ or the combined use of BC with GA or SH is recommended for clinical and laboratory use.

Original languageEnglish
Pages (from-to)531-538
Number of pages8
JournalThe International journal of prosthodontics
Volume21
Issue number6
Publication statusPublished - 2008
Externally publishedYes

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Disinfection
Tooth
Benzalkonium Compounds
Sodium Hypochlorite
Glutaral
Agar
Culture Techniques
Water
Methicillin-Resistant Staphylococcus aureus
Streptococcus
Staphylococcus
Candida
Pseudomonas aeruginosa
Brain

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Clinical evaluation of the efficacy of removing microorganisms to disinfect patient-derived dental impressions. / Egusa, Hiroshi; Watamoto, Takao; Matsumoto, Takuya; Abe, Keiko; Kobayashi, Munemasa; Akashi, Yoshihiro; Yatani, Hirofumi.

In: The International journal of prosthodontics, Vol. 21, No. 6, 2008, p. 531-538.

Research output: Contribution to journalArticle

Egusa, Hiroshi ; Watamoto, Takao ; Matsumoto, Takuya ; Abe, Keiko ; Kobayashi, Munemasa ; Akashi, Yoshihiro ; Yatani, Hirofumi. / Clinical evaluation of the efficacy of removing microorganisms to disinfect patient-derived dental impressions. In: The International journal of prosthodontics. 2008 ; Vol. 21, No. 6. pp. 531-538.
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