Clinical analysis of bacterial strain profiles isolated from urinary tract infections: A 30-year study

Koichiro Wada, Shinya Uehara, Masumi Yamamoto, Takuya Sadahira, Ritsuko Mitsuhata, Motoo Araki, Yasuyuki Kobayashi, Ayano Ishii, Reiko Kariyama, Toyohiko Watanabe, Yasutomo Nasu, Hiromi Kumon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives: We analyzed bacterial strains isolated from urine samples of patients with urinary tract infections (UTI) at Okayama University Hospital over a 30-year period to characterize trends in species and antimicrobial susceptibilities. Methods: Clinical isolates were collected from in- and out-patients with pyuria and bacteriuria who were treated between 1984 and 2014 (one episode per patient and plural isolates were counted in polymicrobial infection). We examined these isolates to identify pathogens and tested for antimicrobial susceptibility. Results: Isolates from complicated UTI over a 30-year period revealed Pseudomonas aeruginosa (P. aeruginosa) was the most frequently isolated in the first decade (1984-1994), MRSA in the second decade (1995-2004), and Escherichia coli (E. coli) in the latest decade (2005-2014). In uncomplicated UTI examined over 20 years, E. coli was the most frequently isolated species accounting for 47-94% of isolates. Fluoroquinolone (FQs)-insusceptible E. coli were first isolated in 1994 and increased to about 35% in 2013 in patients with complicated UTI. Conclusions: Complicated UTI involving P. aeruginosa and MRSA decreased over the last 10 years. Our data suggest that several factors such as shorter hospitalizations, shorter indwelling catheter use, and appropriate antimicrobial use has decreased colonization of P. aeruginosa and MRSA with relative increases in isolation of E. coli including FQs-insusceptible strains.We must continue our surveillance of antimicrobial-resistant bacteria isolated from urine samples and evaluate antibiograms, since their persistence in the urinary tract would be problematic.

Original languageEnglish
JournalJournal of Infection and Chemotherapy
DOIs
Publication statusAccepted/In press - Feb 13 2016

Fingerprint

Urinary Tract Infections
Methicillin-Resistant Staphylococcus aureus
Pseudomonas aeruginosa
Escherichia coli
Urine
Pyuria
Bacteriuria
Indwelling Catheters
Fluoroquinolones
Microbial Sensitivity Tests
Urinary Tract
Coinfection
Hospitalization
Outpatients
Bacteria

Keywords

  • Antibiogram
  • Antimicrobial susceptibility
  • Fluoroquinolone-insusceptible Escherichia coli
  • Urinary tract infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Clinical analysis of bacterial strain profiles isolated from urinary tract infections : A 30-year study. / Wada, Koichiro; Uehara, Shinya; Yamamoto, Masumi; Sadahira, Takuya; Mitsuhata, Ritsuko; Araki, Motoo; Kobayashi, Yasuyuki; Ishii, Ayano; Kariyama, Reiko; Watanabe, Toyohiko; Nasu, Yasutomo; Kumon, Hiromi.

In: Journal of Infection and Chemotherapy, 13.02.2016.

Research output: Contribution to journalArticle

@article{81c76f78700b4b89804701b1f65e3a4e,
title = "Clinical analysis of bacterial strain profiles isolated from urinary tract infections: A 30-year study",
abstract = "Objectives: We analyzed bacterial strains isolated from urine samples of patients with urinary tract infections (UTI) at Okayama University Hospital over a 30-year period to characterize trends in species and antimicrobial susceptibilities. Methods: Clinical isolates were collected from in- and out-patients with pyuria and bacteriuria who were treated between 1984 and 2014 (one episode per patient and plural isolates were counted in polymicrobial infection). We examined these isolates to identify pathogens and tested for antimicrobial susceptibility. Results: Isolates from complicated UTI over a 30-year period revealed Pseudomonas aeruginosa (P. aeruginosa) was the most frequently isolated in the first decade (1984-1994), MRSA in the second decade (1995-2004), and Escherichia coli (E. coli) in the latest decade (2005-2014). In uncomplicated UTI examined over 20 years, E. coli was the most frequently isolated species accounting for 47-94{\%} of isolates. Fluoroquinolone (FQs)-insusceptible E. coli were first isolated in 1994 and increased to about 35{\%} in 2013 in patients with complicated UTI. Conclusions: Complicated UTI involving P. aeruginosa and MRSA decreased over the last 10 years. Our data suggest that several factors such as shorter hospitalizations, shorter indwelling catheter use, and appropriate antimicrobial use has decreased colonization of P. aeruginosa and MRSA with relative increases in isolation of E. coli including FQs-insusceptible strains.We must continue our surveillance of antimicrobial-resistant bacteria isolated from urine samples and evaluate antibiograms, since their persistence in the urinary tract would be problematic.",
keywords = "Antibiogram, Antimicrobial susceptibility, Fluoroquinolone-insusceptible Escherichia coli, Urinary tract infection",
author = "Koichiro Wada and Shinya Uehara and Masumi Yamamoto and Takuya Sadahira and Ritsuko Mitsuhata and Motoo Araki and Yasuyuki Kobayashi and Ayano Ishii and Reiko Kariyama and Toyohiko Watanabe and Yasutomo Nasu and Hiromi Kumon",
year = "2016",
month = "2",
day = "13",
doi = "10.1016/j.jiac.2016.04.004",
language = "English",
journal = "Journal of Infection and Chemotherapy",
issn = "1341-321X",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Clinical analysis of bacterial strain profiles isolated from urinary tract infections

T2 - A 30-year study

AU - Wada, Koichiro

AU - Uehara, Shinya

AU - Yamamoto, Masumi

AU - Sadahira, Takuya

AU - Mitsuhata, Ritsuko

AU - Araki, Motoo

AU - Kobayashi, Yasuyuki

AU - Ishii, Ayano

AU - Kariyama, Reiko

AU - Watanabe, Toyohiko

AU - Nasu, Yasutomo

AU - Kumon, Hiromi

PY - 2016/2/13

Y1 - 2016/2/13

N2 - Objectives: We analyzed bacterial strains isolated from urine samples of patients with urinary tract infections (UTI) at Okayama University Hospital over a 30-year period to characterize trends in species and antimicrobial susceptibilities. Methods: Clinical isolates were collected from in- and out-patients with pyuria and bacteriuria who were treated between 1984 and 2014 (one episode per patient and plural isolates were counted in polymicrobial infection). We examined these isolates to identify pathogens and tested for antimicrobial susceptibility. Results: Isolates from complicated UTI over a 30-year period revealed Pseudomonas aeruginosa (P. aeruginosa) was the most frequently isolated in the first decade (1984-1994), MRSA in the second decade (1995-2004), and Escherichia coli (E. coli) in the latest decade (2005-2014). In uncomplicated UTI examined over 20 years, E. coli was the most frequently isolated species accounting for 47-94% of isolates. Fluoroquinolone (FQs)-insusceptible E. coli were first isolated in 1994 and increased to about 35% in 2013 in patients with complicated UTI. Conclusions: Complicated UTI involving P. aeruginosa and MRSA decreased over the last 10 years. Our data suggest that several factors such as shorter hospitalizations, shorter indwelling catheter use, and appropriate antimicrobial use has decreased colonization of P. aeruginosa and MRSA with relative increases in isolation of E. coli including FQs-insusceptible strains.We must continue our surveillance of antimicrobial-resistant bacteria isolated from urine samples and evaluate antibiograms, since their persistence in the urinary tract would be problematic.

AB - Objectives: We analyzed bacterial strains isolated from urine samples of patients with urinary tract infections (UTI) at Okayama University Hospital over a 30-year period to characterize trends in species and antimicrobial susceptibilities. Methods: Clinical isolates were collected from in- and out-patients with pyuria and bacteriuria who were treated between 1984 and 2014 (one episode per patient and plural isolates were counted in polymicrobial infection). We examined these isolates to identify pathogens and tested for antimicrobial susceptibility. Results: Isolates from complicated UTI over a 30-year period revealed Pseudomonas aeruginosa (P. aeruginosa) was the most frequently isolated in the first decade (1984-1994), MRSA in the second decade (1995-2004), and Escherichia coli (E. coli) in the latest decade (2005-2014). In uncomplicated UTI examined over 20 years, E. coli was the most frequently isolated species accounting for 47-94% of isolates. Fluoroquinolone (FQs)-insusceptible E. coli were first isolated in 1994 and increased to about 35% in 2013 in patients with complicated UTI. Conclusions: Complicated UTI involving P. aeruginosa and MRSA decreased over the last 10 years. Our data suggest that several factors such as shorter hospitalizations, shorter indwelling catheter use, and appropriate antimicrobial use has decreased colonization of P. aeruginosa and MRSA with relative increases in isolation of E. coli including FQs-insusceptible strains.We must continue our surveillance of antimicrobial-resistant bacteria isolated from urine samples and evaluate antibiograms, since their persistence in the urinary tract would be problematic.

KW - Antibiogram

KW - Antimicrobial susceptibility

KW - Fluoroquinolone-insusceptible Escherichia coli

KW - Urinary tract infection

UR - http://www.scopus.com/inward/record.url?scp=84969132838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969132838&partnerID=8YFLogxK

U2 - 10.1016/j.jiac.2016.04.004

DO - 10.1016/j.jiac.2016.04.004

M3 - Article

C2 - 27217185

AN - SCOPUS:84969132838

JO - Journal of Infection and Chemotherapy

JF - Journal of Infection and Chemotherapy

SN - 1341-321X

ER -