Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases

Hidetomo Kobayashi, Eizo Takahashi, Keiji Oguma, Yoshio Fujii, Hiroyasu Yamanaka, Tomoe Negishi, Sakae Arimoto, Takao Tsuji, Keinosuke Okamoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Subtilisin-like proteases have been grouped into six families based on a sequence of the catalytic domain. One of the six is the kexin family, of which furin is a representative protease. All members of the kexin family, except one, are from eukaryotes. The one prokaryotic protease is a serine protease of Aeromonas sorbria (ASP). Here, we examined the substrate specificity of ASP based on the cleavage of short peptides. The results showed that ASP preferentially cleaves the peptide bond following two basic residues, one of which is Lys, but not the bond following a single basic residue. This indicates that the tertiary structure around the catalytic domain of ASP resembles, but is not identical to that of furin. Prekallikrein was cleaved into four fragments by ASP, indicating that the protein must be cleaved at specific sequences.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalFEMS Microbiology Letters
Volume256
Issue number1
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Aeromonas
Serine Proteases
Furin
Peptide Hydrolases
Catalytic Domain
Prekallikrein
Subtilisin
Peptides
Substrate Specificity
Eukaryota
Proteins

Keywords

  • Aeromonas
  • Cleavage specificity
  • Kexin family
  • Serine protease
  • Subtilase

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases. / Kobayashi, Hidetomo; Takahashi, Eizo; Oguma, Keiji; Fujii, Yoshio; Yamanaka, Hiroyasu; Negishi, Tomoe; Arimoto, Sakae; Tsuji, Takao; Okamoto, Keinosuke.

In: FEMS Microbiology Letters, Vol. 256, No. 1, 03.2006, p. 165-170.

Research output: Contribution to journalArticle

Kobayashi, Hidetomo ; Takahashi, Eizo ; Oguma, Keiji ; Fujii, Yoshio ; Yamanaka, Hiroyasu ; Negishi, Tomoe ; Arimoto, Sakae ; Tsuji, Takao ; Okamoto, Keinosuke. / Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases. In: FEMS Microbiology Letters. 2006 ; Vol. 256, No. 1. pp. 165-170.
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AU - Yamanaka, Hiroyasu

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