Classification of the intrafusal muscle fibres in the frog muscle spindle: Histochemical and immunofluorescent studies

A. Yoshimura, N. Fujitsuka, M. Sokabe, K. Naruse, K. Nomura, F. H. Diwan, F. Ito

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intrafusal muscle fibers from bull-frog semitendinosus, iliofibularis and sartorius muscles were classified into three types using the histochemical, immunofluorescent and morphological characteristics, with reference to the extrafusal muscle fibres, which were classified into five types in accordance with Rowlerson and Spurway (1988). Immunofluorescent reactions with antibodies against slow or fast myosins obtained from anterior or posterior latissimus dorsi muscles (ALD or PLD), respectively, of chicken were used as the primary criterion. Histochemical profiles of muscle fibres were classified into nine types of myosin ATPase activity as the secondary criterion. Anti-PLD intrafusal fibres (polar zone) with ATPase profiles of moderate to high acid and alkaline stabilities correspond to large nuclear bag fibres in the classification of Diwan and Ito (1989), whereas anti-ALD fibres (polar zone) with alkaline-labile ATPase profiles of moderate to low acid stability and moderate to high alkaline stability seem to correspond to two types of small nuclear chain fibre. Variations between muscles, between intra- and extrafusal fibres and also between zones along intrafusal fibres are discussed.

Original languageEnglish
Pages (from-to)89-101
Number of pages13
JournalJournal of Anatomy
Volume172
Publication statusPublished - Nov 8 1990
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Ecology, Evolution, Behavior and Systematics
  • Histology
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Yoshimura, A., Fujitsuka, N., Sokabe, M., Naruse, K., Nomura, K., Diwan, F. H., & Ito, F. (1990). Classification of the intrafusal muscle fibres in the frog muscle spindle: Histochemical and immunofluorescent studies. Journal of Anatomy, 172, 89-101.