Classification of Clostridium butyricum based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and pulsed-field gel electrophoresis

Kenji Yokota, Yukako Fujinaga, Kaoru Inoue, Satoshi Shimazaki, Genichirou Seo, Kouichi Takeshi, Eiko Nagamachi, Keiji Oguma

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Eleven strains of Clostridium butyricum collected from different sources were analysed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and pulsed-field gel electrophoresis (PFGE). The strains could be classified into four groups based on their banding profiles of the proteins extracted from the cells on SDS-PAGE. Group I consisted of seven strains, and these strains were further divided into five subgroups by PFGE. The strains belonging to groups II, III, and IV on SDS-PAGE were also classified into the same II to IV groups by PFGE. These data indicate that grouping of the strains of C. butyricum can be performed by employing both SDS-PAGE and PFGE.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
JournalAnaerobe
Volume4
Issue number4
DOIs
Publication statusPublished - Aug 1998

Keywords

  • Classification
  • Clostridium butyricum
  • DNA homology
  • Pulsed-field gel electrophoresis
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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