TY - JOUR
T1 - Classification of Clostridium butyricum based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and pulsed-field gel electrophoresis
AU - Yokota, Kenji
AU - Fujinaga, Yukako
AU - Inoue, Kaoru
AU - Shimazaki, Satoshi
AU - Seo, Genichirou
AU - Takeshi, Kouichi
AU - Nagamachi, Eiko
AU - Oguma, Keiji
PY - 1998/8
Y1 - 1998/8
N2 - Eleven strains of Clostridium butyricum collected from different sources were analysed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and pulsed-field gel electrophoresis (PFGE). The strains could be classified into four groups based on their banding profiles of the proteins extracted from the cells on SDS-PAGE. Group I consisted of seven strains, and these strains were further divided into five subgroups by PFGE. The strains belonging to groups II, III, and IV on SDS-PAGE were also classified into the same II to IV groups by PFGE. These data indicate that grouping of the strains of C. butyricum can be performed by employing both SDS-PAGE and PFGE.
AB - Eleven strains of Clostridium butyricum collected from different sources were analysed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and pulsed-field gel electrophoresis (PFGE). The strains could be classified into four groups based on their banding profiles of the proteins extracted from the cells on SDS-PAGE. Group I consisted of seven strains, and these strains were further divided into five subgroups by PFGE. The strains belonging to groups II, III, and IV on SDS-PAGE were also classified into the same II to IV groups by PFGE. These data indicate that grouping of the strains of C. butyricum can be performed by employing both SDS-PAGE and PFGE.
KW - Classification
KW - Clostridium butyricum
KW - DNA homology
KW - Pulsed-field gel electrophoresis
KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0032144364&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032144364&partnerID=8YFLogxK
U2 - 10.1006/anae.1998.0163
DO - 10.1006/anae.1998.0163
M3 - Article
C2 - 16887639
AN - SCOPUS:0032144364
VL - 4
SP - 177
EP - 181
JO - Anaerobe
JF - Anaerobe
SN - 1075-9964
IS - 4
ER -