Classification of 'activation' antibodies against integrin β1 chain

Jun Tsuchida, Shoko Ueki, Yuji Saito, Junichi Takagi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We compared the effects of two anti-β1 integrin activating antibodies, TS2/16 and AG89, on K562 cell adhesion to fibronectin. Though both antibodies effectively induced cell adhesion, the EC50 for AG89 was more than 200-fold higher than that for TS2/16. Scatchard analysis of the data from [125I]Fab fragment binding to the cells revealed that the TS2/16 epitope is exposed constitutively on all the pi integrin molecules, while only 3% of the β1 integrins on resting K562 cells bear the AG89 epitope. Calculation of the actual number of each antibody bound to the cell during the cell adhesion assay revealed that induction of cell adhesion can be accomplished by binding much fewer AG89 molecules compared to TS2/16. Thus, AG89 and TS2/16 represent distinct classes of anti-integrin activating antibodies that show completely different binding characteristics as well as different activation effects on the integrin molecule upon binding.

Original languageEnglish
Pages (from-to)212-216
Number of pages5
JournalFEBS Letters
Volume416
Issue number2
DOIs
Publication statusPublished - Oct 20 1997
Externally publishedYes

Fingerprint

Integrins
Cell adhesion
Cell Adhesion
Chemical activation
Antibodies
K562 Cells
Molecules
Epitopes
Immunoglobulin Fab Fragments
Fibronectins
Assays

Keywords

  • Cell adhesion
  • Fc receptor
  • Integrin
  • Stimulatory antibody

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Classification of 'activation' antibodies against integrin β1 chain. / Tsuchida, Jun; Ueki, Shoko; Saito, Yuji; Takagi, Junichi.

In: FEBS Letters, Vol. 416, No. 2, 20.10.1997, p. 212-216.

Research output: Contribution to journalArticle

Tsuchida, Jun ; Ueki, Shoko ; Saito, Yuji ; Takagi, Junichi. / Classification of 'activation' antibodies against integrin β1 chain. In: FEBS Letters. 1997 ; Vol. 416, No. 2. pp. 212-216.
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