Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma

Koji Uotani, Tomohiro Fujiwara, Aki Yoshida, Shintaro Iwata, Takuya Morita, Masahiro Kiyono, Suguru Yokoo, Toshiyuki Kunisada, Ken Takeda, Jou Hasei, Kunihiko Numoto, Yutaka Nezu, Tsukasa Yonemoto, Takeshi Ishii, Akira Kawai, Takahiro Ochiya, Toshihumi Ozaki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) patients remains unknown. Initial global miRNA screening using SS patient serum and SS cell culture media identified a signature of four upregulated miRNAs. Among these candidates, miR-92b-3p secretion from SS cells was confirmed, which was embedded within tumour-derived exosomes rather than argonaute-2. Animal experiments revealed a close correlation between serum miR-92b-3p levels and tumour dynamics. Clinical relevance was validated in two independent clinical cohorts, and we subsequently identified that serum miR-92b-3p levels were significantly higher in SS patients in comparison to that in healthy individuals. Moreover, serum miR-92b-3p was robust in discriminating patients with SS from the other STS patients and reflected tumour burden in SS patients. Overall, liquid biopsy using serum miR-92b-3p expression levels may represent a novel approach for monitoring tumour dynamics of SS.

Original languageEnglish
Article number14634
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Synovial Sarcoma
MicroRNAs
Biomarkers
Serum
Sarcoma
Exosomes
Neoplasms
Body Fluids
Tumor Burden
Culture Media
Cell Culture Techniques
Biopsy

ASJC Scopus subject areas

  • General

Cite this

Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma. / Uotani, Koji; Fujiwara, Tomohiro; Yoshida, Aki; Iwata, Shintaro; Morita, Takuya; Kiyono, Masahiro; Yokoo, Suguru; Kunisada, Toshiyuki; Takeda, Ken; Hasei, Jou; Numoto, Kunihiko; Nezu, Yutaka; Yonemoto, Tsukasa; Ishii, Takeshi; Kawai, Akira; Ochiya, Takahiro; Ozaki, Toshihumi.

In: Scientific Reports, Vol. 7, No. 1, 14634, 01.12.2017.

Research output: Contribution to journalArticle

Uotani, K, Fujiwara, T, Yoshida, A, Iwata, S, Morita, T, Kiyono, M, Yokoo, S, Kunisada, T, Takeda, K, Hasei, J, Numoto, K, Nezu, Y, Yonemoto, T, Ishii, T, Kawai, A, Ochiya, T & Ozaki, T 2017, 'Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma', Scientific Reports, vol. 7, no. 1, 14634. https://doi.org/10.1038/s41598-017-12660-5
Uotani K, Fujiwara T, Yoshida A, Iwata S, Morita T, Kiyono M et al. Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma. Scientific Reports. 2017 Dec 1;7(1). 14634. https://doi.org/10.1038/s41598-017-12660-5
Uotani, Koji ; Fujiwara, Tomohiro ; Yoshida, Aki ; Iwata, Shintaro ; Morita, Takuya ; Kiyono, Masahiro ; Yokoo, Suguru ; Kunisada, Toshiyuki ; Takeda, Ken ; Hasei, Jou ; Numoto, Kunihiko ; Nezu, Yutaka ; Yonemoto, Tsukasa ; Ishii, Takeshi ; Kawai, Akira ; Ochiya, Takahiro ; Ozaki, Toshihumi. / Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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