@article{96747905b0e2470eafda95eb500bc365,
title = "Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma",
abstract = "The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) patients remains unknown. Initial global miRNA screening using SS patient serum and SS cell culture media identified a signature of four upregulated miRNAs. Among these candidates, miR-92b-3p secretion from SS cells was confirmed, which was embedded within tumour-derived exosomes rather than argonaute-2. Animal experiments revealed a close correlation between serum miR-92b-3p levels and tumour dynamics. Clinical relevance was validated in two independent clinical cohorts, and we subsequently identified that serum miR-92b-3p levels were significantly higher in SS patients in comparison to that in healthy individuals. Moreover, serum miR-92b-3p was robust in discriminating patients with SS from the other STS patients and reflected tumour burden in SS patients. Overall, liquid biopsy using serum miR-92b-3p expression levels may represent a novel approach for monitoring tumour dynamics of SS.",
author = "Koji Uotani and Tomohiro Fujiwara and Aki Yoshida and Shintaro Iwata and Takuya Morita and Masahiro Kiyono and Suguru Yokoo and Toshiyuki Kunisada and Ken Takeda and Jou Hasei and Kunihiko Numoto and Yutaka Nezu and Tsukasa Yonemoto and Takeshi Ishii and Akira Kawai and Takahiro Ochiya and Toshifumi Ozaki",
note = "Funding Information: The authors thank all patients who agreed to this study project. The authors also acknowledge the National Cancer Center Biobank, Tsukasa Yonemoto and Takeshi Ishii at the Division of Orthopaedic Surgery of Chiba Cancer Center for providing the serum samples. We also thanks to Norifumi Naka at the Department of Orthopaedic Surgery of Osaka University, and Akira Ogose at the Department of Orthopaedic Surgery of Nigata University, for providing SS and MFS cell lines, and the Central Research Laboratory of the Okayama University Medical School for the technical support for the extraction and validation of exosomes.The authors are supported by the Grant-in-Aid of Japan Orthopaedics and Traumatology Research Foundation, Inc. No. 311, the NOVARTIS Foundation for the Promotion of Science, JSPS KAKENHI Grant Number 16K20054, and JSPS KAKENHI Grant Number 16H05449. Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41598-017-12660-5",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}