Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors.

Aiqun Li, Masaki Saito, Jen Zen Chuang, Yun Yu Tseng, Carlos Dedesma, Kazuhito Tomizawa, Taku Kaitsuka, Ching Hwa Sung

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Primary cilia are displayed during the G(0)/G(1) phase of many cell types. Cilia are resorbed as cells prepare to re-enter the cell cycle, but the causal and molecular link between these two cellular events remains unclear. We show that Tctex-1 phosphorylated at Thr 94 is recruited to ciliary transition zones before S-phase entry and has a pivotal role in both ciliary disassembly and cell cycle progression. However, the role of Tctex-1 in S-phase entry is dispensable in non-ciliated cells. Exogenously adding a phospho-mimic Tctex-1(T94E) mutant accelerates cilium disassembly and S-phase entry. These results support a model in which the cilia act as a brake to prevent cell cycle progression. Mechanistic studies show the involvement of actin dynamics in Tctex-1-regulated cilium resorption. Tctex-1 phosphorylated at Thr 94 is also selectively enriched at the ciliary transition zones of cortical neural progenitors, and has a key role in controlling G(1) length, cell cycle entry and fate determination of these cells during corticogenesis.

Original languageEnglish
Pages (from-to)402-411
Number of pages10
JournalNature Cell Biology
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 1 2011
Externally publishedYes

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Cilia
S Phase
Cell Cycle
Actins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors. / Li, Aiqun; Saito, Masaki; Chuang, Jen Zen; Tseng, Yun Yu; Dedesma, Carlos; Tomizawa, Kazuhito; Kaitsuka, Taku; Sung, Ching Hwa.

In: Nature Cell Biology, Vol. 13, No. 4, 01.04.2011, p. 402-411.

Research output: Contribution to journalArticle

Li, A, Saito, M, Chuang, JZ, Tseng, YY, Dedesma, C, Tomizawa, K, Kaitsuka, T & Sung, CH 2011, 'Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors.', Nature Cell Biology, vol. 13, no. 4, pp. 402-411. https://doi.org/10.1038/ncb2218
Li, Aiqun ; Saito, Masaki ; Chuang, Jen Zen ; Tseng, Yun Yu ; Dedesma, Carlos ; Tomizawa, Kazuhito ; Kaitsuka, Taku ; Sung, Ching Hwa. / Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors. In: Nature Cell Biology. 2011 ; Vol. 13, No. 4. pp. 402-411.
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