Chronic treatment with amlodipine modulates adrenocortical angiotensin II receptors in spontaneously hypertensive rats

Masayuki Kishida, Fumio Otsuka, Toshio Ogura, Hideo Kataoka, Yoshio Nakamura, Takayoshi Yamauchi, Masami Takahashi, Kazuaki Yokota, Yukari Mimura, Hirofumi Makino

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of long-term treatment with calcium-antagonist, amlodipine, on angiotensin II receptors in the adrenal cortex of spontaneously hypertensive rats (SHR). Seven-week-old male SHR were treated with oral amlodipine (10 mg/kg/day) or vehicle (saline) for four weeks. Age-matched normotensive Wistar-Kyoto (WKY) rats were treated with the vehicle similar to control SHR. Systolic blood pressure (SBP) showed time-dependent increase in SHR but not in WKY rats, while amlodipine treatment significantly reduced the high SBP in SHR. Plasma renin activity was serially increased in SHR, which was further enhanced by amlodipine treatment. But the plasma aldosterone level which was increased in SHR was not changed by amlodipine. Competitive reverse transcriptase-polymerase chain reaction showed that the level of adrenocortical angiotensin II type 1 receptor (AT1R) mRNA progressively decreased in vehicle-treated SHR compared to WKY rats and that 4-week course of amlodipine treatment significantly increased AT1R mRNA in SHR to levels comparable to those in WKY rats. Amlodipine treatment reduced the level of adrenocortical angiotensin II type 2 receptor (AT2R) mRNA in SHR from 8 weeks of age. Thus, chronic amlodipine treatment differently modulates both adrenocortical AT1R and AT2R in SHR in a possibly direct manner.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalLife Sciences
Volume69
Issue number1
DOIs
Publication statusPublished - May 25 2001

Fingerprint

Amlodipine
Angiotensin Receptors
Inbred SHR Rats
Rats
Inbred WKY Rats
Angiotensin Type 1 Receptor
Angiotensin Type 2 Receptor
Blood Pressure
Messenger RNA
Blood pressure
Adrenal Cortex
Aldosterone
Reverse Transcriptase Polymerase Chain Reaction
Renin
Plasmas
Polymerase chain reaction
RNA-Directed DNA Polymerase

Keywords

  • Adrenal cortex
  • AT1-receptor
  • AT2-receptor
  • Hypertension and aldosterone

ASJC Scopus subject areas

  • Pharmacology

Cite this

Chronic treatment with amlodipine modulates adrenocortical angiotensin II receptors in spontaneously hypertensive rats. / Kishida, Masayuki; Otsuka, Fumio; Ogura, Toshio; Kataoka, Hideo; Nakamura, Yoshio; Yamauchi, Takayoshi; Takahashi, Masami; Yokota, Kazuaki; Mimura, Yukari; Makino, Hirofumi.

In: Life Sciences, Vol. 69, No. 1, 25.05.2001, p. 113-122.

Research output: Contribution to journalArticle

Kishida, Masayuki ; Otsuka, Fumio ; Ogura, Toshio ; Kataoka, Hideo ; Nakamura, Yoshio ; Yamauchi, Takayoshi ; Takahashi, Masami ; Yokota, Kazuaki ; Mimura, Yukari ; Makino, Hirofumi. / Chronic treatment with amlodipine modulates adrenocortical angiotensin II receptors in spontaneously hypertensive rats. In: Life Sciences. 2001 ; Vol. 69, No. 1. pp. 113-122.
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