Chronic forced swim stress of rats increases frontal cortical 5-HT2 receptors and the wet-dog shakes they mediate, but not frontal cortical β-adrenoceptors

Katsuyuki Takao, Tadashi Nagatani, Yoshihisa Kitamura, Koh Kawasaki, Hiroshi Hayakawa, Shigeto Yamawaki

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50 Citations (Scopus)


We studied the effects of chronic forced swim stress on 5-HT2 receptors and β-adrenoceptors in the rat frontal cortex. The number of 5-HT2 receptors was increased immediately after the last chronic stress, but not after an acute stress. In vivo, the number of wet-dog shakes induced by a 5-HT2 receptor agonist, (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), was increased 24 h after the last chronic stress. However, the concentrations of 5-HT and 5-hydroxyindole acetic acid (5-HIAA), measured by high pressure liquid chromatography (HPLC), were not altered by this stress. Binding sites for [3H]CGP-12177, i.e., β-adrenoceptor sites, were unchanged after both the acute and the chronic stress. These results suggest that, in the rat, the chronic forced swim stress increases the number of frontal cortical 5-HT2 receptors and the number of wet-dog shakes mediated by these receptors, while the number of frontal cortical β-adrenoceptors is not increased by this treatment.

Original languageEnglish
Pages (from-to)721-726
Number of pages6
JournalEuropean Journal of Pharmacology
Issue number2-3
Publication statusPublished - Dec 29 1995
Externally publishedYes



  • 5-HT receptor
  • Forced swim test
  • Stress
  • Wet-dog shake
  • β-Adrenoceptor

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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