Abstract
Background: Epidemiologic studies suggest a relationship between periodontitis and liver diseases. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and liver diseases. Methods: Fourteen male Wistar rats (8 weeks old) were divided into two groups: a periodontitis group in which Escherichia coli lipopolysaccharide (LPS) and Streptomyces griseus proteases were applied into the gingival sulcus for 8 weeks, and a control group using pyrogen-free water instead. After blood samples were collected, periodontal tissues and liver specimens were analyzed. Results: Chronic administration of LPS and proteases to the gingival sulcus induced periodontitis and liver injury, including steatosis with inflammation and sinusoidal fibrosis. Apoptosis, enhanced concentration of 8-hydroxydeoxyguanosine, and activated production of tumor necrosis factor-alpha in liver were observed in the periodontitis group, with increased gingival inflammation, serum LPS, and reactive oxygen species. Conclusion: Periodontal inflammation in a rat model induced fatty liver disease through increased serum LPS.
| Original language | English |
|---|---|
| Pages (from-to) | 1999-2006 |
| Number of pages | 8 |
| Journal | Journal of Periodontology |
| Volume | 78 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2007 |
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Keywords
- Animals
- Fatty liver
- Hepatic steatosis
- Lipopolysaccharides
- Periodontitis
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Chronic administration of lipopolysaccharide and proteases induces periodontal inflammation and hepatic steatosis in rats. / Tomofuji, Takaaki; Ekuni, Daisuke; Yamanaka, Reiko; Kusano, Hiroki; Azuma, Tetsuji; Sanbe, Toshihiro; Tamaki, Naofumi; Yamamoto, Tatsuo; Watanabe, Tatsuo; Miyauchi, Mutsumi; Takata, Takashi.
In: Journal of Periodontology, Vol. 78, No. 10, 10.2007, p. 1999-2006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chronic administration of lipopolysaccharide and proteases induces periodontal inflammation and hepatic steatosis in rats
AU - Tomofuji, Takaaki
AU - Ekuni, Daisuke
AU - Yamanaka, Reiko
AU - Kusano, Hiroki
AU - Azuma, Tetsuji
AU - Sanbe, Toshihiro
AU - Tamaki, Naofumi
AU - Yamamoto, Tatsuo
AU - Watanabe, Tatsuo
AU - Miyauchi, Mutsumi
AU - Takata, Takashi
PY - 2007/10
Y1 - 2007/10
N2 - Background: Epidemiologic studies suggest a relationship between periodontitis and liver diseases. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and liver diseases. Methods: Fourteen male Wistar rats (8 weeks old) were divided into two groups: a periodontitis group in which Escherichia coli lipopolysaccharide (LPS) and Streptomyces griseus proteases were applied into the gingival sulcus for 8 weeks, and a control group using pyrogen-free water instead. After blood samples were collected, periodontal tissues and liver specimens were analyzed. Results: Chronic administration of LPS and proteases to the gingival sulcus induced periodontitis and liver injury, including steatosis with inflammation and sinusoidal fibrosis. Apoptosis, enhanced concentration of 8-hydroxydeoxyguanosine, and activated production of tumor necrosis factor-alpha in liver were observed in the periodontitis group, with increased gingival inflammation, serum LPS, and reactive oxygen species. Conclusion: Periodontal inflammation in a rat model induced fatty liver disease through increased serum LPS.
AB - Background: Epidemiologic studies suggest a relationship between periodontitis and liver diseases. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and liver diseases. Methods: Fourteen male Wistar rats (8 weeks old) were divided into two groups: a periodontitis group in which Escherichia coli lipopolysaccharide (LPS) and Streptomyces griseus proteases were applied into the gingival sulcus for 8 weeks, and a control group using pyrogen-free water instead. After blood samples were collected, periodontal tissues and liver specimens were analyzed. Results: Chronic administration of LPS and proteases to the gingival sulcus induced periodontitis and liver injury, including steatosis with inflammation and sinusoidal fibrosis. Apoptosis, enhanced concentration of 8-hydroxydeoxyguanosine, and activated production of tumor necrosis factor-alpha in liver were observed in the periodontitis group, with increased gingival inflammation, serum LPS, and reactive oxygen species. Conclusion: Periodontal inflammation in a rat model induced fatty liver disease through increased serum LPS.
KW - Animals
KW - Fatty liver
KW - Hepatic steatosis
KW - Lipopolysaccharides
KW - Periodontitis
UR - http://www.scopus.com/inward/record.url?scp=35648939246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35648939246&partnerID=8YFLogxK
U2 - 10.1902/jop.2007.070056
DO - 10.1902/jop.2007.070056
M3 - Article
C2 - 17916001
AN - SCOPUS:35648939246
VL - 78
SP - 1999
EP - 2006
JO - Journal of Periodontology
JF - Journal of Periodontology
SN - 0022-3492
IS - 10
ER -