Chromosome partitioning in Escherichia coli: Novel mutants producing anucleate cells

S. Hiraga, H. Niki, T. Ogura, C. Ichinose, H. Mori, B. Ezaki, A. Jaffe

Research output: Contribution to journalArticle

269 Citations (Scopus)

Abstract

To study the chromosomal partitioning mechanism in cell division, we have isolated a novel type of Escherichia coli mutants which formed anucleate cells, by using newly developed techniques. One of them, named mukA1, is not lethal and produces normal-sized anucleate cells at a frequency of 0.5 to 3% of total cells in exponentially growing populations but does not produce filamentous cells. Results suggest that the mutant is defective in the chromosome positioning at regular intracellular positions and fails frequency to partition the replicated daughter chromosomes into both daughter cells, resulting in production of one anucleate daughter cell and one with two chromosomes. The mukA1 mutation causes pleiotropic effects: slow growth, hypersensitivity to sodium dodecyl sulfate, and tolerance to colicin E1 protein, in addition to anucleate cell formation. Cloning of the mukA gene indicates that the mukA1 mutation is recessive and that the mukA gene is identical to the tolC gene coding for an outer membrane protein.

Original languageEnglish
Pages (from-to)1496-1505
Number of pages10
JournalJournal of Bacteriology
Volume171
Issue number3
Publication statusPublished - 1989
Externally publishedYes

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Chromosomes
Escherichia coli
Chromosome Positioning
Colicins
Recessive Genes
Mutation
Sodium Dodecyl Sulfate
Cell Division
Genes
Organism Cloning
Hypersensitivity
Membrane Proteins
Growth
Population
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Hiraga, S., Niki, H., Ogura, T., Ichinose, C., Mori, H., Ezaki, B., & Jaffe, A. (1989). Chromosome partitioning in Escherichia coli: Novel mutants producing anucleate cells. Journal of Bacteriology, 171(3), 1496-1505.

Chromosome partitioning in Escherichia coli : Novel mutants producing anucleate cells. / Hiraga, S.; Niki, H.; Ogura, T.; Ichinose, C.; Mori, H.; Ezaki, B.; Jaffe, A.

In: Journal of Bacteriology, Vol. 171, No. 3, 1989, p. 1496-1505.

Research output: Contribution to journalArticle

Hiraga, S, Niki, H, Ogura, T, Ichinose, C, Mori, H, Ezaki, B & Jaffe, A 1989, 'Chromosome partitioning in Escherichia coli: Novel mutants producing anucleate cells', Journal of Bacteriology, vol. 171, no. 3, pp. 1496-1505.
Hiraga S, Niki H, Ogura T, Ichinose C, Mori H, Ezaki B et al. Chromosome partitioning in Escherichia coli: Novel mutants producing anucleate cells. Journal of Bacteriology. 1989;171(3):1496-1505.
Hiraga, S. ; Niki, H. ; Ogura, T. ; Ichinose, C. ; Mori, H. ; Ezaki, B. ; Jaffe, A. / Chromosome partitioning in Escherichia coli : Novel mutants producing anucleate cells. In: Journal of Bacteriology. 1989 ; Vol. 171, No. 3. pp. 1496-1505.
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